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- PDB-1sfs: 1.07 A crystal structure of an uncharacterized B. stearothermophi... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1sfs | ||||||
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Title | 1.07 A crystal structure of an uncharacterized B. stearothermophilus protein | ||||||
![]() | Hypothetical protein | ||||||
![]() | Structural Genomics / unknown function / possible bacterial Lysozyme / PSI / Protein Structure Initiative / Midwest Center for Structural Genomics / MCSG | ||||||
Function / homology | Rv2525c-like, glycoside hydrolase-like domain / Rv2525c-like, glycoside hydrolase-like domain / Glycosidases / Glycoside hydrolase superfamily / TIM Barrel / Alpha-Beta Barrel / Alpha Beta / PHOSPHATE ION / Rbstp1166 protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Minasov, G. / Brunzelle, J.S. / Shuvalova, L. / Moy, S.F. / Collart, F.R. / Anderson, W.F. / Midwest Center for Structural Genomics (MCSG) | ||||||
![]() | ![]() Title: 1.07 A crystal structure of an uncharacterized B. stearothermophilus protein Authors: Minasov, G. / Brunzelle, J.S. / Shuvalova, L. / Moy, S.F. / Collart, F.R. / Anderson, W.F. | ||||||
History |
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Remark 999 | SEQUENCE RESIDUES 0 TO -23 ARE HIS TAG RESIDUES. THE SEQUENCE OF THE PROTEIN HAS NOT YET BEEN ...SEQUENCE RESIDUES 0 TO -23 ARE HIS TAG RESIDUES. THE SEQUENCE OF THE PROTEIN HAS NOT YET BEEN DEPOSITED IN ANY SEQUENCE REFERENCE DATABASE. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 127 KB | Display | ![]() |
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PDB format | ![]() | 105.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 435 KB | Display | ![]() |
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Full document | ![]() | 441.3 KB | Display | |
Data in XML | ![]() | 15.5 KB | Display | |
Data in CIF | ![]() | 24.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26965.385 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: RBSTP1166 / Plasmid: pMCSG7 / Species (production host): Escherichia coli / Production host: ![]() ![]() |
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#2: Chemical | ChemComp-PO4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.19 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 0.2M Ammonium Phosphate, 20% PEG, 0.1M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jun 27, 2003 |
Radiation | Monochromator: Si 111 Channel / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.07→15 Å / Num. all: 90332 / Num. obs: 90332 / % possible obs: 94.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 8.53 % / Rmerge(I) obs: 0.059 / Net I/σ(I): 20.24 |
Reflection shell | Resolution: 1.07→1.11 Å / Redundancy: 5.4 % / Rmerge(I) obs: 0.287 / Mean I/σ(I) obs: 6.64 / Num. unique all: 7244 / % possible all: 73.7 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 16.84 Å2 | |||||||||||||||||||||||||||||||||
Refine analyze | Num. disordered residues: 205 / Occupancy sum hydrogen: 1570.71 / Occupancy sum non hydrogen: 1975.72 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.07→15 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.07→1.11 Å
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