+Open data
-Basic information
Entry | Database: PDB / ID: 1sf5 | ||||||
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Title | Structure of oxidized state of the P94A mutant of amicyanin | ||||||
Components | Amicyanin | ||||||
Keywords | ELECTRON TRANSPORT / blue copper protein / beta sandwich | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Paracoccus denitrificans (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 1.1 Å | ||||||
Authors | Carrell, C.J. / Sun, D. / Jiang, S. / Davidson, V.L. / Mathews, F.S. | ||||||
Citation | Journal: Biochemistry / Year: 2004 Title: Structural Studies of Two Mutants of Amicyanin from Paracoccus denitrificans That Stabilize the Reduced State of the Copper. Authors: Carrell, C.J. / Sun, D. / Jiang, S. / Davidson, V.L. / Mathews, F.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sf5.cif.gz | 61.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sf5.ent.gz | 43.8 KB | Display | PDB format |
PDBx/mmJSON format | 1sf5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1sf5_validation.pdf.gz | 412.2 KB | Display | wwPDB validaton report |
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Full document | 1sf5_full_validation.pdf.gz | 413.6 KB | Display | |
Data in XML | 1sf5_validation.xml.gz | 8.3 KB | Display | |
Data in CIF | 1sf5_validation.cif.gz | 11.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sf/1sf5 ftp://data.pdbj.org/pub/pdb/validation_reports/sf/1sf5 | HTTPS FTP |
-Related structure data
Related structure data | 1sf3C 1sfdC 1sfhC 1aacS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 11495.134 Da / Num. of mol.: 1 / Mutation: P94A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Paracoccus denitrificans (bacteria) / Gene: MAUC, AMI / Production host: Escherichia coli (E. coli) / References: UniProt: P22364 |
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#2: Chemical | ChemComp-CU / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.85 Å3/Da / Density % sol: 33.39 % |
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Crystal grow | Temperature: 293 K / Method: evaporation / pH: 5 Details: 3.6 M monobasic sodium phosphate, 0.4 M dibasic potassium phosphate, pH 5.0, EVAPORATION, temperature 293.0K |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 14-BM-C / Wavelength: 0.9 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Aug 14, 2002 |
Radiation | Monochromator: Bent Ge 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 1.1→30 Å / Num. all: 32824 / Num. obs: 32824 / % possible obs: 96.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.7 % / Rsym value: 0.056 / Net I/σ(I): 24.5 |
Reflection shell | Resolution: 1.1→1.12 Å / Mean I/σ(I) obs: 2.8 / Rsym value: 0.323 / % possible all: 71.5 |
-Processing
Software |
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Refinement | Method to determine structure: FOURIER SYNTHESIS Starting model: PDB file 1AAC Resolution: 1.1→30 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.1→30 Å
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