+Open data
-Basic information
Entry | Database: PDB / ID: 1sdb | ||||||
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Title | PORCINE DESB1-2 DESPENTAPEPTIDE(B26-B30) INSULIN | ||||||
Components | (DESB1-2 DESPENTAPEPTIDE (B26-B30) INSULIN) x 2 | ||||||
Keywords | HORMONE | ||||||
Function / homology | Function and homology information Insulin processing / IRS activation / Signal attenuation / Insulin receptor signalling cascade / Signaling by Insulin receptor / Synthesis, secretion, and deacylation of Ghrelin / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / Insulin receptor recycling / glycoprotein biosynthetic process / response to L-arginine ...Insulin processing / IRS activation / Signal attenuation / Insulin receptor signalling cascade / Signaling by Insulin receptor / Synthesis, secretion, and deacylation of Ghrelin / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / Insulin receptor recycling / glycoprotein biosynthetic process / response to L-arginine / positive regulation of lipoprotein lipase activity / lactate biosynthetic process / positive regulation of fatty acid biosynthetic process / positive regulation of glucose metabolic process / lipoprotein biosynthetic process / COPI-mediated anterograde transport / lipid biosynthetic process / positive regulation of DNA replication / negative regulation of gluconeogenesis / positive regulation of insulin receptor signaling pathway / positive regulation of protein autophosphorylation / insulin-like growth factor receptor binding / positive regulation of protein secretion / hormone activity / glucose metabolic process / glucose homeostasis / insulin receptor signaling pathway / positive regulation of cell migration / extracellular space / identical protein binding Similarity search - Function | ||||||
Biological species | Sus scrofa (pig) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.65 Å | ||||||
Authors | Liang, D.-C. / Diao, J.-S. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1997 Title: Structure of monomeric porcine DesB1-B2 despentapeptide (B26-B30) insulin at 1.65 A resolution. Authors: Diao, J.S. / Wan, Z.L. / Chang, W.R. / Liang, D.C. #1: Journal: Sci.Sin.(Engl.Ed.) / Year: 1987 Title: Refinement of the Structure of Despentapeptide (B26-B30) Insulin at 1.5A Resolution Authors: Dai, J.B. / Lou, M.Z. / You, J.M. / Liang, D.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sdb.cif.gz | 18.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sdb.ent.gz | 11.8 KB | Display | PDB format |
PDBx/mmJSON format | 1sdb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1sdb_validation.pdf.gz | 408.6 KB | Display | wwPDB validaton report |
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Full document | 1sdb_full_validation.pdf.gz | 408.9 KB | Display | |
Data in XML | 1sdb_validation.xml.gz | 4.3 KB | Display | |
Data in CIF | 1sdb_validation.cif.gz | 5.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sd/1sdb ftp://data.pdbj.org/pub/pdb/validation_reports/sd/1sdb | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein/peptide | Mass: 2383.698 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / Organ: PANCREAS / References: UniProt: P01315 |
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#2: Protein/peptide | Mass: 2595.972 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / Organ: PANCREAS / References: UniProt: P01315 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.8 Å3/Da / Density % sol: 32 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 8.4 / Method: unknown | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Jun 1, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→27.3 Å / Num. obs: 3829 / % possible obs: 87.1 % / Observed criterion σ(I): 0 / Redundancy: 4 % / Rmerge(I) obs: 0.0272 |
-Processing
Software |
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Refinement | Resolution: 1.65→10 Å / σ(F): 2 Details: THE ELECTRON DENSITY FOR THE DESB1-2 DESPENTAPEPTIDE (B 26 - B 30) INSULIN IS ALMOST COMPLETELY DEFINED WHILE DENSITY FOR THE SIDE CHAIN OF RESIDUE ASN B 3 IS WEAK.
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Displacement parameters | Biso mean: 20.04 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.65→10 Å
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Refine LS restraints |
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Software | *PLUS Name: GPRLSA / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.197 / Rfactor Rfree: 0.2203 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |