+Open data
-Basic information
Entry | Database: PDB / ID: 1s4h | ||||||
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Title | NMR structure of cross-reactive peptides from L. braziliensis | ||||||
Components | 60S acidic ribosomal protein P2 | ||||||
Keywords | RIBOSOME / Leishmania brazilienses / antigenic peptide / ribosomal p2 protein | ||||||
Function / homology | Trypanosoma cruzi ribosomal protein P2-like / Ribosomal protein P2 / Ribosomal protein L12/P1/P2 family / Ribosomal protein P1/P2, N-terminal domain / cytoplasmic translational elongation / 60s Acidic ribosomal protein / cytosolic large ribosomal subunit / structural constituent of ribosome / Large ribosomal subunit protein P2 Function and homology information | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Soares, M.R. / Bisch, P.M. / Campos de Carvalho, A.C. / Valente, A.P. / Almeida, F.C.L. | ||||||
Citation | Journal: Febs Lett. / Year: 2004 Title: Correlation between conformation and antibody binding: NMR structure of cross-reactive peptides from T. cruzi, human and L. braziliensis Authors: Soares, M.R. / Bisch, P.M. / Campos De Carvalho, A.C. / Valente, A.P. / Almeida, F.C.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1s4h.cif.gz | 55 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1s4h.ent.gz | 37.5 KB | Display | PDB format |
PDBx/mmJSON format | 1s4h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1s4h_validation.pdf.gz | 334.8 KB | Display | wwPDB validaton report |
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Full document | 1s4h_full_validation.pdf.gz | 395.2 KB | Display | |
Data in XML | 1s4h_validation.xml.gz | 5.5 KB | Display | |
Data in CIF | 1s4h_validation.cif.gz | 8.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/s4/1s4h ftp://data.pdbj.org/pub/pdb/validation_reports/s4/1s4h | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1460.477 Da / Num. of mol.: 1 / Fragment: a13 - C-terminal domain / Source method: obtained synthetically Details: peptide obtained by solid phase synthesis. the sequence is naturally found in Leishmania braziliensis. References: UniProt: O44010 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details | Contents: 2mM of peptide; 10% D2O; 10mM phosphate buffer at pH 5.5 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 10mM phosphate buffer / pH: 5.5 / Pressure: ambient / Temperature: 278 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |