[English] 日本語
Yorodumi- PDB-1qw1: Solution Structure of the C-Terminal Domain of DtxR residues 110-226 -
+Open data
-Basic information
Entry | Database: PDB / ID: 1qw1 | ||||||
---|---|---|---|---|---|---|---|
Title | Solution Structure of the C-Terminal Domain of DtxR residues 110-226 | ||||||
Components | Diphtheria toxin repressor | ||||||
Keywords | GENE REGULATION / REPRESSOR / DTXR / C-TERMINAL DOMAIN / PROKARYOTIC SH3 DOMAIN / TRANSCRIPTION REGULATION / PEPTIDE-BINDING | ||||||
Function / homology | Function and homology information transition metal ion binding / SH3 domain binding / protein dimerization activity / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA binding / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Corynebacterium diphtheriae (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model type details | minimized average | ||||||
Authors | Wylie, G.P. / Rangachari, V. / Bienkiewicz, E.A. / Love, J.F. / Murphy, J.R. / Logan, T.M. | ||||||
Citation | Journal: Biochemistry / Year: 2005 Title: Prolylpeptide binding by the prokaryotic SH3-like domain of the diphtheria toxin repressor: a regulatory switch. Authors: Wylie, G.P. / Rangachari, V. / Bienkiewicz, E.A. / Marin, V. / Bhattacharya, N. / Love, J.F. / Murphy, J.R. / Logan, T.M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1qw1.cif.gz | 519.1 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1qw1.ent.gz | 429.5 KB | Display | PDB format |
PDBx/mmJSON format | 1qw1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1qw1_validation.pdf.gz | 341.2 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1qw1_full_validation.pdf.gz | 487.3 KB | Display | |
Data in XML | 1qw1_validation.xml.gz | 42.6 KB | Display | |
Data in CIF | 1qw1_validation.cif.gz | 55.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qw/1qw1 ftp://data.pdbj.org/pub/pdb/validation_reports/qw/1qw1 | HTTPS FTP |
-Related structure data
Related structure data | 1qvpC C: citing same article (ref.) |
---|---|
Similar structure data | |
Other databases |
|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 13339.945 Da / Num. of mol.: 1 / Fragment: RESIDUES 110-226 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Corynebacterium diphtheriae (bacteria) / Gene: DTXR / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL-21(DE3) / References: UniProt: P33120, UniProt: P0DJL7*PLUS |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||||||||||
NMR details | Text: Please see primary citation for more details on the experiments recorded. |
-Sample preparation
Details |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software | Name: CNS / Version: 1 / Developer: Brunger / Classification: refinement |
---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: minimized average structure |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 60 / Conformers submitted total number: 14 |