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- PDB-1pxy: Crystal structure of the actin-crosslinking core of Arabidopsis f... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1pxy | ||||||
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Title | Crystal structure of the actin-crosslinking core of Arabidopsis fimbrin | ||||||
![]() | fimbrin-like protein | ||||||
![]() | STRUCTURAL PROTEIN / CALPONIN HOMOLOGY / F-ACTIN-BINDING DOMAIN (ABD) / F-ACTIN-CROSSLINKING / Structural Genomics / PSI / Protein Structure Initiative / New York SGX Research Center for Structural Genomics / NYSGXRC | ||||||
Function / homology | ![]() actin filament network formation / actin filament bundle / actin filament bundle assembly / actin filament / circadian rhythm / actin filament binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Klein, M.G. / Shi, W. / Tseng, Y. / Wirtz, D. / Almo, S.C. / Burley, S.K. / New York SGX Research Center for Structural Genomics (NYSGXRC) | ||||||
![]() | ![]() Title: Structure of the actin crosslinking core of fimbrin. Authors: Klein, M.G. / Shi, W. / Ramagopal, U. / Tseng, Y. / Wirtz, D. / Kovar, D.R. / Staiger, C.J. / Almo, S.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 198.6 KB | Display | ![]() |
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PDB format | ![]() | 156.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1rt8C ![]() 1aoaS S: Starting model for refinement C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 57547.645 Da / Num. of mol.: 2 / Fragment: actin-crosslinking core, residues 123-623 / Mutation: V240L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.53 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8.2 Details: 6% PEG 8000, 100 mM Tris, 1mM DTT, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 200 K | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 1, 2001 | |||||||||||||||
Radiation | Monochromator: Double flat crystal monochromator with fixed exit geometry Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.4→30 Å / Num. all: 51046 / Num. obs: 44865 / % possible obs: 87.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.97 % / Rsym value: 0.067 / Net I/σ(I): 20.71 | |||||||||||||||
Reflection shell | Resolution: 2.4→2.49 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.315 / Mean I/σ(I) obs: 3.35 / Num. unique all: 4394 / Rsym value: 0.303 / % possible all: 86.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1AOA Resolution: 2.4→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.4→20 Å
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LS refinement shell | Resolution: 2.4→2.49 Å
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