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Open data
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Basic information
Entry | Database: PDB / ID: 1pv6 | ||||||
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Title | Crystal structure of lactose permease | ||||||
![]() | Lactose permease | ||||||
![]() | TRANSPORT PROTEIN / Transport / Sugar transport / Symport / membrane protein | ||||||
Function / homology | ![]() lactose:proton symporter activity / lactose transport / cytidine transmembrane transporter activity / carbohydrate:proton symporter activity / uridine transmembrane transporter activity / lactose binding / : / carbohydrate transport / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Abramson, J. / Smirnova, I. / Kasho, V. / Verner, G. / Kaback, H.R. / Iwata, S. | ||||||
![]() | ![]() Title: Structure and mechanism of the lactose permease of Escherichia coli Authors: Abramson, J. / Smirnova, I. / Kasho, V. / Verner, G. / Kaback, H.R. / Iwata, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 166.2 KB | Display | ![]() |
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PDB format | ![]() | 135.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 378.4 KB | Display | ![]() |
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Full document | ![]() | 464.6 KB | Display | |
Data in XML | ![]() | 28.8 KB | Display | |
Data in CIF | ![]() | 41.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 46484.797 Da / Num. of mol.: 2 / Mutation: C154G Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 6.45 Å3/Da | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Mar 28, 2003 |
Radiation | Monochromator: Si 111 / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.009 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→40 Å / Num. all: 31004 / Num. obs: 25733 / % possible obs: 83 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -1 |
Reflection shell | Resolution: 3.5→3.6 Å / % possible all: 71.8 |
Reflection | *PLUS Num. obs: 25917 / % possible obs: 85.9 % / Redundancy: 2.9 % / Num. measured all: 63863 / Rmerge(I) obs: 0.075 |
Reflection shell | *PLUS % possible obs: 71.8 % / Rmerge(I) obs: 0.461 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 3.5→40 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 40 Å / % reflection Rfree: 5 % | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 3.5 Å / Lowest resolution: 3.6 Å / Rfactor Rfree: 0.379 / Rfactor Rwork: 0.356 |