[English] 日本語
![](img/lk-miru.gif)
- PDB-1pa3: Crystal Structure of Glutathione-S-transferase from Plasmodium fa... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1pa3 | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal Structure of Glutathione-S-transferase from Plasmodium falciparum | ||||||
![]() | Glutathione s-transferase, putative | ||||||
![]() | TRANSFERASE | ||||||
Function / homology | ![]() Heme degradation / Paracetamol ADME / Glutathione conjugation / Synthesis of Prostaglandins (PG) and Thromboxanes (TX) / Azathioprine ADME / Detoxification of Reactive Oxygen Species / Neutrophil degranulation / glutathione transferase / glutathione transferase activity / glutathione metabolic process Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Perbandt, M. / Betzel, C. / Liebau, E. | ||||||
![]() | ![]() Title: Native and inhibited structure of a Mu class-related glutathione S-transferase from Plasmodium falciparum Authors: Perbandt, M. / Burmeister, C. / Walter, R.D. / Betzel, C. / Liebau, E. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 90.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 70.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 434.1 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 445 KB | Display | |
Data in XML | ![]() | 16.7 KB | Display | |
Data in CIF | ![]() | 22.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 24817.039 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.08 Å3/Da / Density % sol: 40.85 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: Ammonium sulfate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6 / Method: vapor diffusion, hanging drop / Details: Burmeister, C., (2003) Acta Cryst., D59, 1469. | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction source | Source: ![]() |
---|---|
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Mar 20, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→50 Å / Num. obs: 13330 / Observed criterion σ(I): 0 |
Reflection shell | Highest resolution: 2.6 Å / % possible all: 97.92 |
Reflection | *PLUS Num. obs: 13027 / % possible obs: 97.6 % / Redundancy: 6 % / Rmerge(I) obs: 0.13 |
Reflection shell | *PLUS Lowest resolution: 2.69 Å / % possible obs: 94.7 % / Redundancy: 5.6 % / Rmerge(I) obs: 0.519 / Mean I/σ(I) obs: 2.3 |
-
Processing
Software | Name: REFMAC / Version: 5 / Classification: refinement | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→15 Å
| ||||||||||||||||||||
Software | *PLUS Name: REFMAC / Version: 5 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.6 Å / Num. reflection Rfree: 658 / % reflection Rfree: 5 % / Rfactor Rfree: 0.258 / Rfactor Rwork: 0.205 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
| ||||||||||||||||||||
LS refinement shell | *PLUS Rfactor Rfree: 0.351 / Rfactor Rwork: 0.322 |