+Open data
-Basic information
Entry | Database: PDB / ID: 1p0u | ||||||||||||||||||
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Title | Sheared G/C Base Pair | ||||||||||||||||||
Components | 5'-D(*Keywords | DNA / Sheared GC base pair / single-residue loop / syn cytidine | Function / homology | DNA / DNA (> 10) | Function and homology information Method | SOLUTION NMR / distance geometry, simulated annealing, molecular dynamics | Authors | Chin, K.H. / Chou, S.H. | Citation | Journal: J.Mol.Biol. / Year: 2003 | Title: Sheared-type G(anti).C(syn) Base-pair: A Unique d(GXC) Loop Closure Motif. Authors: Chin, K.H. / Chou, S.H. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1p0u.cif.gz | 85.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1p0u.ent.gz | 67.4 KB | Display | PDB format |
PDBx/mmJSON format | 1p0u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1p0u_validation.pdf.gz | 297.2 KB | Display | wwPDB validaton report |
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Full document | 1p0u_full_validation.pdf.gz | 342.5 KB | Display | |
Data in XML | 1p0u_validation.xml.gz | 2.5 KB | Display | |
Data in CIF | 1p0u_validation.cif.gz | 3.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/p0/1p0u ftp://data.pdbj.org/pub/pdb/validation_reports/p0/1p0u | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: DNA chain | Mass: 3976.599 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details | Contents: 20mM NaCl, 10mM phosphate buffer / Solvent system: 90% H2O, 10% D2O; 100% D2O |
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Sample conditions | pH: 6.8 / Pressure: ambient / Temperature: 278 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Manufacturer: Varian / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: distance geometry, simulated annealing, molecular dynamics Software ordinal: 1 | ||||||||
NMR representative | Selection criteria: lowest energy | ||||||||
NMR ensemble | Conformers submitted total number: 10 |