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Open data
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Basic information
Entry | Database: PDB / ID: 1oj6 | ||||||
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Title | Human brain neuroglobin three-dimensional structure | ||||||
![]() | Neuroglobin | ||||||
![]() | OXYGEN TRANSPORT / NEUROGLOBIN / HEME HEXACOORDINATION | ||||||
Function / homology | ![]() Intracellular oxygen transport / GDP-dissociation inhibitor activity / nitrite reductase activity / Oxidoreductases; Acting on other nitrogenous compounds as donors / oxygen transport / oxygen carrier activity / oxygen binding / cellular response to hypoxia / response to hypoxia / mitochondrial matrix ...Intracellular oxygen transport / GDP-dissociation inhibitor activity / nitrite reductase activity / Oxidoreductases; Acting on other nitrogenous compounds as donors / oxygen transport / oxygen carrier activity / oxygen binding / cellular response to hypoxia / response to hypoxia / mitochondrial matrix / heme binding / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pesce, A. / Dewilde, S. / Nardini, M. / Moens, L. / Ascenzi, P. / Hankeln, T. / Burmester, T. / Bolognesi, M. | ||||||
![]() | ![]() Title: Human Brain Neuroglobin Structure Reveals a Distinct Mode of Controlling Oxygen Affinity Authors: Pesce, A. / Dewilde, S. / Nardini, M. / Moens, L. / Ascenzi, P. / Hankeln, T. / Burmester, T. / Bolognesi, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 133.4 KB | Display | ![]() |
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PDB format | ![]() | 105.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.9 MB | Display | ![]() |
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Full document | ![]() | 1.9 MB | Display | |
Data in XML | ![]() | 28 KB | Display | |
Data in CIF | ![]() | 37 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16873.150 Da / Num. of mol.: 4 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-HEM / #3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | Compound details | FUNCTION: INVOLVED IN OXYGEN TRANSPORT IN THE BRAIN. ENGINEERED MUTATION IN CHAINS A, B, C, D, CYS ...FUNCTION: INVOLVED IN OXYGEN TRANSPORT IN THE BRAIN. ENGINEERED | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 36 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 Details: 1.4 M AMMONIUM SULPHATE, 3% ISOPROPANOL, 0.05 M SODIUM CITRATE, PH 6.5 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 277 K / pH: 6.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Date: Mar 15, 2002 | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.95→40 Å / Num. obs: 35871 / % possible obs: 98.5 % / Redundancy: 2.6 % / Rmerge(I) obs: 0.055 / Net I/σ(I): 17 | ||||||||||||
Reflection shell | Resolution: 1.95→1.98 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.22 / Mean I/σ(I) obs: 5 / % possible all: 97.9 | ||||||||||||
Reflection | *PLUS Highest resolution: 1.95 Å / Lowest resolution: 40 Å / Redundancy: 2.6 % / Num. measured all: 92865 / Rmerge(I) obs: 0.055 | ||||||||||||
Reflection shell | *PLUS % possible obs: 97.9 % / Redundancy: 2.5 % / Rmerge(I) obs: 0.22 / Mean I/σ(I) obs: 5 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 26 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→40 Å
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Refinement | *PLUS Lowest resolution: 40 Å / % reflection Rfree: 10 % | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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