ONLY PROTEASE DOMAIN OF THE ASSEMBLIN IS PRESENT IN THE CRYSTAL.THE SEQUENCE IS MODIFIED AT THE N- ...ONLY PROTEASE DOMAIN OF THE ASSEMBLIN IS PRESENT IN THE CRYSTAL.THE SEQUENCE IS MODIFIED AT THE N-TERMINUS DUE TO THE INTRODUCTION OF A THROMBIN CLEAVAGE SITE.
手法: 蒸気拡散法, ハンギングドロップ法 / pH: 4.6 詳細: HANGING DROP METHOD USING A RESERVOIR SOLUTION OF 1.25 - 1.5 M SODIUM FORMATE,100 MM SODIUM CITRATE PH4.6 5 MM EDTA, PROTEIN IN 100 MM NACL 20 MM THRIS-HCL PH 7.5 1 MM EDTA 10 MM BETA-MERCAPTOETHANOL
解像度: 2.3→42 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 0 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 0 詳細: REFINED USING THE TWINNED TARGET, TWINNING FRACTION 0.477, OPERATOR -H,-K,L THE REFINEMENT HAS BEEN CARRIED OUT AGAINST TWINNED DATA USING THE CORRESPONDING TARGET IN CNS. R-FACTORS CAN NOT ...詳細: REFINED USING THE TWINNED TARGET, TWINNING FRACTION 0.477, OPERATOR -H,-K,L THE REFINEMENT HAS BEEN CARRIED OUT AGAINST TWINNED DATA USING THE CORRESPONDING TARGET IN CNS. R-FACTORS CAN NOT DIRECTLY BE COMPARED TO THE ONES FROM UNTWINNED STRUCTURES. FOR MAP CALCULATIONS, A DIFFERENT DATASET WITH A TWINNING FRACTION MORE DIFFERENT FROM 0.5 HAS BEEN USED. ITS RESOLUTION WAS LIMITED TO 2.5 A. SOME REGIONS CARACTERISED BY B FACTORS ABOVE 90 A2 ARE POORLY DEFINED.