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- PDB-1o4p: CRYSTAL STRUCTURE OF SH2 IN COMPLEX WITH RU78791. -

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Basic information

Entry
Database: PDB / ID: 1o4p
TitleCRYSTAL STRUCTURE OF SH2 IN COMPLEX WITH RU78791.
ComponentsPROTO-ONCOGENE TYROSINE-PROTEIN KINASE SRC
KeywordsSIGNALING PROTEIN / SH2 DOMAIN FRAGMENT APPROACH
Function / homology
Function and homology information


: / primary ovarian follicle growth / regulation of caveolin-mediated endocytosis / regulation of toll-like receptor 3 signaling pathway / regulation of cell projection assembly / positive regulation of ovarian follicle development / positive regulation of platelet-derived growth factor receptor-beta signaling pathway / cellular response to prolactin / positive regulation of male germ cell proliferation / dendritic filopodium ...: / primary ovarian follicle growth / regulation of caveolin-mediated endocytosis / regulation of toll-like receptor 3 signaling pathway / regulation of cell projection assembly / positive regulation of ovarian follicle development / positive regulation of platelet-derived growth factor receptor-beta signaling pathway / cellular response to prolactin / positive regulation of male germ cell proliferation / dendritic filopodium / negative regulation of telomere maintenance / response to mineralocorticoid / positive regulation of dephosphorylation / ERBB2 signaling pathway / Regulation of commissural axon pathfinding by SLIT and ROBO / regulation of epithelial cell migration / positive regulation of protein transport / Regulation of gap junction activity / BMP receptor binding / cellular response to progesterone stimulus / Activated NTRK2 signals through FYN / negative regulation of focal adhesion assembly / skeletal muscle cell proliferation / regulation of vascular permeability / positive regulation of protein processing / positive regulation of integrin activation / Netrin mediated repulsion signals / regulation of intracellular estrogen receptor signaling pathway / intestinal epithelial cell development / Co-stimulation by CD28 / positive regulation of glucose metabolic process / transcytosis / osteoclast development / cellular response to fluid shear stress / Activated NTRK3 signals through PI3K / connexin binding / response to acidic pH / focal adhesion assembly / signal complex assembly / adherens junction organization / positive regulation of small GTPase mediated signal transduction / branching involved in mammary gland duct morphogenesis / Regulation of RUNX1 Expression and Activity / positive regulation of Ras protein signal transduction / positive regulation of podosome assembly / DCC mediated attractive signaling / EPH-Ephrin signaling / regulation of bone resorption / positive regulation of lamellipodium morphogenesis / Ephrin signaling / cellular response to peptide hormone stimulus / myoblast proliferation / odontogenesis / negative regulation of mitochondrial depolarization / Signal regulatory protein family interactions / podosome / cellular response to fatty acid / MET activates PTK2 signaling / Regulation of KIT signaling / postsynaptic specialization, intracellular component / regulation of early endosome to late endosome transport / leukocyte migration / Signaling by ALK / GP1b-IX-V activation signalling / Fc-gamma receptor signaling pathway involved in phagocytosis / phospholipase activator activity / Co-inhibition by CTLA4 / Receptor Mediated Mitophagy / oogenesis / EPHA-mediated growth cone collapse / DNA biosynthetic process / positive regulation of Notch signaling pathway / interleukin-6-mediated signaling pathway / p130Cas linkage to MAPK signaling for integrins / positive regulation of smooth muscle cell migration / Signaling by EGFR / stress fiber assembly / dendritic growth cone / stimulatory C-type lectin receptor signaling pathway / regulation of cell-cell adhesion / RUNX2 regulates osteoblast differentiation / regulation of heart rate by cardiac conduction / Recycling pathway of L1 / uterus development / negative regulation of intrinsic apoptotic signaling pathway / phospholipase binding / neurotrophin TRK receptor signaling pathway / PECAM1 interactions / GRB2:SOS provides linkage to MAPK signaling for Integrins / RHOU GTPase cycle / platelet-derived growth factor receptor signaling pathway / Long-term potentiation / RET signaling / negative regulation of anoikis / FCGR activation / positive regulation of epithelial cell migration / ephrin receptor signaling pathway / EPH-ephrin mediated repulsion of cells / GAB1 signalosome / negative regulation of hippo signaling
Similarity search - Function
SH2 domain / SHC Adaptor Protein / : / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / Src homology 3 domains / SH2 domain superfamily ...SH2 domain / SHC Adaptor Protein / : / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
2-PHENYLMALONIC ACID / Proto-oncogene tyrosine-protein kinase Src
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / molecular replacement / Resolution: 1.9 Å
AuthorsLange, G. / Loenze, P. / Liesum, A.
CitationJournal: J.Med.Chem. / Year: 2003
Title: Requirements for specific binding of low affinity inhibitor fragments to the SH2 domain of (pp60)Src are identical to those for high affinity binding of full length inhibitors.
Authors: Lange, G. / Lesuisse, D. / Deprez, P. / Schoot, B. / Loenze, P. / Benard, D. / Marquette, J.P. / Broto, P. / Sarubbi, E. / Mandine, E.
History
DepositionJun 15, 2003Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 17, 2004Provider: repository / Type: Initial release
Revision 1.1Apr 26, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Aug 16, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: PROTO-ONCOGENE TYROSINE-PROTEIN KINASE SRC
hetero molecules


Theoretical massNumber of molelcules
Total (without water)12,5552
Polymers12,3751
Non-polymers1801
Water2,216123
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)26.836, 59.066, 64.922
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein PROTO-ONCOGENE TYROSINE-PROTEIN KINASE SRC / P60-SRC / C-SRC


Mass: 12374.964 Da / Num. of mol.: 1 / Fragment: SH2 DOMAIN
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SRC / Plasmid: BL21 (DE3) / Production host: Escherichia coli (E. coli) / References: UniProt: P12931, EC: 2.7.1.112
#2: Chemical ChemComp-791 / 2-PHENYLMALONIC ACID / RU78191


Mass: 180.157 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C9H8O4
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 123 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.2 Å3/Da / Density % sol: 41.9 %
Crystal growpH: 5.5 / Details: pH 5.50
Crystal grow
*PLUS
pH: 5.5 / Method: vapor diffusion, sitting drop / Details: Lesuisse, D., (2002) J.Med.Chem., 45, 2379.
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-IDDetails
115 %PEG4001reservoir
210 %DMSO1reservoir
3100 mMMES1reservoirpH5.5
410 mMdithiothreitol1reservoir
560-80 mg/mlprotein1drop
610 mMdithiothreitol1droppH5.5

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: ROTATING ANODE / Type: ELLIOTT GX-21 / Wavelength: 1.5418
DetectorType: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Apr 14, 1998
RadiationMonochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionResolution: 1.9→40 Å / Num. obs: 8412 / % possible obs: 97.7 % / Observed criterion σ(I): -3 / Rmerge(I) obs: 0.101 / Net I/σ(I): 17
Reflection shellResolution: 1.9→1.95 Å / Rmerge(I) obs: 0.228 / Mean I/σ(I) obs: 7 / % possible all: 96.8
Reflection
*PLUS
Rmerge(I) obs: 0.098

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Processing

Software
NameVersionClassification
XDSdata scaling
XDSdata reduction
X-PLOR3.851model building
X-PLOR3.851refinement
X-PLOR3.851phasing
RefinementMethod to determine structure: molecular replacement
Starting model: 1SHD

1shd


Resolution: 1.9→8 Å / Data cutoff high absF: 1000000 / Data cutoff low absF: 0.1
RfactorNum. reflection% reflection
Rwork0.169 --
obs0.169 8412 97.7 %
Displacement parametersBiso mean: 22.6 Å2
Refinement stepCycle: LAST / Resolution: 1.9→8 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms849 0 13 123 985
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONx_bond_d0.014
X-RAY DIFFRACTIONx_bond_d_na
X-RAY DIFFRACTIONx_bond_d_prot
X-RAY DIFFRACTIONx_angle_d
X-RAY DIFFRACTIONx_angle_d_na
X-RAY DIFFRACTIONx_angle_d_prot
X-RAY DIFFRACTIONx_angle_deg1.4
X-RAY DIFFRACTIONx_angle_deg_na
X-RAY DIFFRACTIONx_angle_deg_prot
X-RAY DIFFRACTIONx_dihedral_angle_d
X-RAY DIFFRACTIONx_dihedral_angle_d_na
X-RAY DIFFRACTIONx_dihedral_angle_d_prot
X-RAY DIFFRACTIONx_improper_angle_d
X-RAY DIFFRACTIONx_improper_angle_d_na
X-RAY DIFFRACTIONx_improper_angle_d_prot
X-RAY DIFFRACTIONx_mcbond_it
X-RAY DIFFRACTIONx_mcangle_it
X-RAY DIFFRACTIONx_scbond_it
X-RAY DIFFRACTIONx_scangle_it

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