+Open data
-Basic information
Entry | Database: PDB / ID: 1ntq | ||||||
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Title | 5'(dCCUCCUU)3':3'(rAGGAGGAAA)5' | ||||||
Components |
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Keywords | DNA/RNA / DNA / RNA / hybrid / propynyl / DNA-RNA COMPLEX | ||||||
Function / homology | DNA / RNA Function and homology information | ||||||
Method | SOLUTION NMR / simulated annealing, energy minimization | ||||||
Model type details | minimized average | ||||||
Authors | Znosko, B.M. / Barnes III, T.W. / Krugh, T.R. / Turner, D.H. | ||||||
Citation | Journal: J.Am.Chem.Soc. / Year: 2003 Title: NMR Studies of DNA Single Strands and DNA:RNA Hybrids With and Without 1-Propynylation at C5 of Oligopyrimidines Authors: Znosko, B.M. / Barnes III, T.W. / Krugh, T.R. / Turner, D.H. #1: Journal: J.Am.Chem.Soc. / Year: 2001 Title: Long-Range Cooperativity in Molecular Recognition of RNA by Oligodeoxynucleotides with Multiple C5-(1-Propynyl) Pyrimidines Authors: Barnes III, T.W. / Turner, D.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ntq.cif.gz | 20.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ntq.ent.gz | 11.9 KB | Display | PDB format |
PDBx/mmJSON format | 1ntq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ntq_validation.pdf.gz | 297.6 KB | Display | wwPDB validaton report |
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Full document | 1ntq_full_validation.pdf.gz | 297.5 KB | Display | |
Data in XML | 1ntq_validation.xml.gz | 3.2 KB | Display | |
Data in CIF | 1ntq_validation.cif.gz | 3.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nt/1ntq ftp://data.pdbj.org/pub/pdb/validation_reports/nt/1ntq | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 2981.895 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: DNA chain | Mass: 1982.270 Da / Num. of mol.: 1 / Source method: obtained synthetically |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D NOESY |
-Sample preparation
Details |
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing, energy minimization / Software ordinal: 1 Details: The structure is based on a total of 140 interproton distance restraints, 18 hydrogen bond restraints, and 104 dihedral angle restraints. | ||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |