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Yorodumi- PDB-1nsa: THREE-DIMENSIONAL STRUCTURE OF PORCINE PROCARBOXYPEPTIDASE B: A S... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1nsa | ||||||
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Title | THREE-DIMENSIONAL STRUCTURE OF PORCINE PROCARBOXYPEPTIDASE B: A STRUCTURAL BASIS OF ITS INACTIVITY | ||||||
Components | PROCARBOXYPEPTIDASE B | ||||||
Keywords | SERINE PROTEASE / PORCINE PROCARBOXYPEPTIDASE | ||||||
Function / homology | Function and homology information carboxypeptidase B / metallocarboxypeptidase activity / cytoplasmic vesicle / proteolysis / extracellular space / zinc ion binding Similarity search - Function | ||||||
Biological species | Sus scrofa (pig) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.3 Å | ||||||
Authors | Huber, R. | ||||||
Citation | Journal: EMBO J. / Year: 1991 Title: Three-dimensional structure of porcine procarboxypeptidase B: a structural basis of its inactivity. Authors: Coll, M. / Guasch, A. / Aviles, F.X. / Huber, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1nsa.cif.gz | 85.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1nsa.ent.gz | 70 KB | Display | PDB format |
PDBx/mmJSON format | 1nsa.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1nsa_validation.pdf.gz | 385.1 KB | Display | wwPDB validaton report |
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Full document | 1nsa_full_validation.pdf.gz | 396.1 KB | Display | |
Data in XML | 1nsa_validation.xml.gz | 10.6 KB | Display | |
Data in CIF | 1nsa_validation.cif.gz | 16.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ns/1nsa ftp://data.pdbj.org/pub/pdb/validation_reports/ns/1nsa | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 44998.777 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: P09955 | ||
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#2: Chemical | ChemComp-ZN / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.75 Å3/Da / Density % sol: 55.29 % |
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Crystal grow | pH: 5 / Details: 1.5 M AMMONIUM SULFATE AT PH5, pH 5.0 |
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 5 / Method: vapor diffusion |
Components of the solutions | *PLUS Conc.: 1.5 M / Common name: ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 277 K |
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Detector | Type: BRUKER NONIUS FAST / Detector: TV AREA DETECTOR |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Highest resolution: 2.3 Å / Num. obs: 23217 / % possible obs: 93.7 % / Rmerge(I) obs: 0.09 |
Reflection | *PLUS Num. measured all: 115799 |
Reflection shell | *PLUS Highest resolution: 2.3 Å / Lowest resolution: 2.4 Å / % possible obs: 72.2 % |
-Processing
Software |
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Refinement | Resolution: 2.3→8 Å /
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Refinement step | Cycle: LAST / Resolution: 2.3→8 Å
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Software | *PLUS Name: EREF / Classification: refinement | ||||||||||||||
Refinement | *PLUS Rfactor obs: 0.169 | ||||||||||||||
Solvent computation | *PLUS | ||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||
Refine LS restraints | *PLUS
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