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Yorodumi- PDB-1ns1: RNA-BINDING DOMAIN OF NON-STRUCTURAL PROTEIN 1 FROM INFLUENZA VIR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ns1 | ||||||
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Title | RNA-BINDING DOMAIN OF NON-STRUCTURAL PROTEIN 1 FROM INFLUENZA VIRUS, NMR, 16 STRUCTURES | ||||||
Components | NONSTRUCTURAL PROTEIN 1 | ||||||
Keywords | NONSTRUCTURAL PROTEIN / RNA-BINDING / SSRNA-BINDING / DSRNA-BINDING / POLYA-RNA-BINDING / DIMERIC SIX HELICAL STRUCTURE | ||||||
Function / homology | Function and homology information symbiont-mediated suppression of host cytokine production / symbiont-mediated suppression of host innate immune response / symbiont-mediated suppression of host mRNA processing / symbiont-mediated suppression of host PKR/eIFalpha signaling / protein serine/threonine kinase inhibitor activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity / double-stranded RNA binding / host cell cytoplasm / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / virus-mediated perturbation of host defense response ...symbiont-mediated suppression of host cytokine production / symbiont-mediated suppression of host innate immune response / symbiont-mediated suppression of host mRNA processing / symbiont-mediated suppression of host PKR/eIFalpha signaling / protein serine/threonine kinase inhibitor activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity / double-stranded RNA binding / host cell cytoplasm / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / virus-mediated perturbation of host defense response / host cell nucleus / identical protein binding Similarity search - Function | ||||||
Biological species | Influenza A virus | ||||||
Method | SOLUTION NMR / VARIABLE TARGET FUNCTION MINIMIZATION | ||||||
Authors | Montelione, G.T. / Chien, C.-Y. / Tejero, R. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1997 Title: A novel RNA-binding motif in influenza A virus non-structural protein 1. Authors: Chien, C.Y. / Tejero, R. / Huang, Y. / Zimmerman, D.E. / Rios, C.B. / Krug, R.M. / Montelione, G.T. #1: Journal: RNA / Year: 1995 Title: An Amino-Terminal Polypeptide Fragment of the Influenza Virus Ns1 Protein Possesses Specific RNA-Binding Activity and Largely Helical Backbone Structure Authors: Qian, X.Y. / Chien, C.Y. / Lu, Y. / Montelione, G.T. / Krug, R.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ns1.cif.gz | 722.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ns1.ent.gz | 609 KB | Display | PDB format |
PDBx/mmJSON format | 1ns1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ns1_validation.pdf.gz | 355.3 KB | Display | wwPDB validaton report |
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Full document | 1ns1_full_validation.pdf.gz | 578.7 KB | Display | |
Data in XML | 1ns1_validation.xml.gz | 42.9 KB | Display | |
Data in CIF | 1ns1_validation.cif.gz | 60.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ns/1ns1 ftp://data.pdbj.org/pub/pdb/validation_reports/ns/1ns1 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8310.420 Da / Num. of mol.: 2 / Fragment: RNA-BINDING DOMAIN, RESIDUES 1 - 73 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus / Genus: Influenzavirus A / Strain: UDORN/307/72 / Cell line: BL21 / Gene: J02169 / Plasmid: PET11A-NS1A(1-73)CC / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P03495 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 6.0 / Temperature: 293 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY 500 / Manufacturer: Varian / Model: UNITY 500 / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: VARIABLE TARGET FUNCTION MINIMIZATION / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | |||||||||
NMR ensemble | Conformer selection criteria: LOWEST RESIDUAL CONSTRAINT VIOLATIONS Conformers calculated total number: 100 / Conformers submitted total number: 16 |