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- PDB-1ns1: RNA-BINDING DOMAIN OF NON-STRUCTURAL PROTEIN 1 FROM INFLUENZA VIR... -

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Basic information

Entry
Database: PDB / ID: 1ns1
TitleRNA-BINDING DOMAIN OF NON-STRUCTURAL PROTEIN 1 FROM INFLUENZA VIRUS, NMR, 16 STRUCTURES
ComponentsNONSTRUCTURAL PROTEIN 1
KeywordsNONSTRUCTURAL PROTEIN / RNA-BINDING / SSRNA-BINDING / DSRNA-BINDING / POLYA-RNA-BINDING / DIMERIC SIX HELICAL STRUCTURE
Function / homology
Function and homology information


symbiont-mediated suppression of host mRNA processing / symbiont-mediated suppression of host PKR/eIFalpha signaling / signaling receptor inhibitor activity / protein serine/threonine kinase inhibitor activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity / double-stranded RNA binding / host cell cytoplasm / symbiont-mediated suppression of host innate immune response / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / symbiont-mediated suppression of host gene expression ...symbiont-mediated suppression of host mRNA processing / symbiont-mediated suppression of host PKR/eIFalpha signaling / signaling receptor inhibitor activity / protein serine/threonine kinase inhibitor activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity / double-stranded RNA binding / host cell cytoplasm / symbiont-mediated suppression of host innate immune response / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / symbiont-mediated suppression of host gene expression / signaling receptor binding / host cell nucleus / identical protein binding
Similarity search - Function
Influenza A virus NS1 protein / Influenza A virus NS1, effector domain-like superfamily / Influenza non-structural protein (NS1) / Influenza non-structural protein (NS1) / S15/NS1, RNA-binding / Helix Hairpins / S15/NS1, RNA-binding / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Non-structural protein 1
Similarity search - Component
Biological speciesInfluenza A virus
MethodSOLUTION NMR / VARIABLE TARGET FUNCTION MINIMIZATION
AuthorsMontelione, G.T. / Chien, C.-Y. / Tejero, R.
Citation
Journal: Nat.Struct.Biol. / Year: 1997
Title: A novel RNA-binding motif in influenza A virus non-structural protein 1.
Authors: Chien, C.Y. / Tejero, R. / Huang, Y. / Zimmerman, D.E. / Rios, C.B. / Krug, R.M. / Montelione, G.T.
#1: Journal: RNA / Year: 1995
Title: An Amino-Terminal Polypeptide Fragment of the Influenza Virus Ns1 Protein Possesses Specific RNA-Binding Activity and Largely Helical Backbone Structure
Authors: Qian, X.Y. / Chien, C.Y. / Lu, Y. / Montelione, G.T. / Krug, R.M.
History
DepositionOct 2, 1997Processing site: BNL
Revision 1.0Jan 14, 1998Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: NONSTRUCTURAL PROTEIN 1
B: NONSTRUCTURAL PROTEIN 1


Theoretical massNumber of molelcules
Total (without water)16,6212
Polymers16,6212
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)16 / 100LOWEST RESIDUAL CONSTRAINT VIOLATIONS
Representative

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Components

#1: Protein NONSTRUCTURAL PROTEIN 1 / NS1


Mass: 8310.420 Da / Num. of mol.: 2 / Fragment: RNA-BINDING DOMAIN, RESIDUES 1 - 73
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Influenza A virus / Genus: Influenzavirus A / Strain: UDORN/307/72 / Cell line: BL21 / Gene: J02169 / Plasmid: PET11A-NS1A(1-73)CC / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P03495

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111MD-TRIPLE RESONANCE NMR EXPERIMENTS
121MD-NOESY EXPERIMENTS
131H/D EXCHANGE EXPERIMENTS

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Sample preparation

Sample conditionspH: 6 / Temperature: 293 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometerType: Varian UNITY 500 / Manufacturer: Varian / Model: UNITY 500 / Field strength: 500 MHz

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Processing

NMR software
NameDeveloperClassification
DIANAWUTHRICHrefinement
DIANAstructure solution
RefinementMethod: VARIABLE TARGET FUNCTION MINIMIZATION / Software ordinal: 1
Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE.
NMR ensembleConformer selection criteria: LOWEST RESIDUAL CONSTRAINT VIOLATIONS
Conformers calculated total number: 100 / Conformers submitted total number: 16

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