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- PDB-1n05: Crystal Structure of Schizosaccharomyces pombe Riboflavin Kinase ... -

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Basic information

Entry
Database: PDB / ID: 1n05
TitleCrystal Structure of Schizosaccharomyces pombe Riboflavin Kinase Reveals a Novel ATP and Riboflavin Binding Fold
Componentsputative Riboflavin kinase
KeywordsTRANSFERASE / kinase / phosphoryl transferases / flavin cofactors / metal binding
Function / homology
Function and homology information


Vitamin B2 (riboflavin) metabolism / riboflavin metabolic process / riboflavin kinase / riboflavin kinase activity / FMN biosynthetic process / riboflavin biosynthetic process / mitochondrial inner membrane / mitochondrion / zinc ion binding / ATP binding ...Vitamin B2 (riboflavin) metabolism / riboflavin metabolic process / riboflavin kinase / riboflavin kinase activity / FMN biosynthetic process / riboflavin biosynthetic process / mitochondrial inner membrane / mitochondrion / zinc ion binding / ATP binding / nucleus / cytosol
Similarity search - Function
Riboflavin kinase domain, bacterial/eukaryotic / Riboflavin kinase / Riboflavin kinase / Riboflavin kinase / Riboflavin kinase-like / Riboflavin kinase domain superfamily / Elongation Factor Tu (Ef-tu); domain 3 / Beta Barrel / Mainly Beta
Similarity search - Domain/homology
Biological speciesSchizosaccharomyces pombe (fission yeast)
MethodX-RAY DIFFRACTION / MIR / Resolution: 2.1 Å
AuthorsBauer, S. / Kemter, K. / Bacher, A. / Huber, R. / Fischer, M. / Steinbacher, S.
CitationJournal: J.Mol.Biol. / Year: 2003
Title: Crystal Structure of Schizosaccharomyces pombe Riboflavin Kinase Reveals a Novel ATP and Riboflavin Binding Fold
Authors: Bauer, S. / Kemter, K. / Bacher, A. / Huber, R. / Fischer, M. / Steinbacher, S.
History
DepositionOct 11, 2002Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 25, 2003Provider: repository / Type: Initial release
Revision 1.1Apr 28, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 14, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: putative Riboflavin kinase


Theoretical massNumber of molelcules
Total (without water)18,9391
Polymers18,9391
Non-polymers00
Water2,342130
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)69.497, 69.497, 139.851
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number178
Space group name H-MP6122

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Components

#1: Protein putative Riboflavin kinase


Mass: 18938.750 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Schizosaccharomyces pombe (fission yeast)
Gene: SPCC18.16C / Plasmid: pNCO / Production host: Escherichia coli (E. coli) / References: UniProt: O74866, riboflavin kinase
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 130 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.57 Å3/Da / Density % sol: 52.19 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Crystal grow
*PLUS
Temperature: 20 ℃ / pH: 8
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-IDDetailsChemical formula
120 mMTris-HCl1droppH8.0
2100 mM1dropNaCl
310 mg/mlprotein1drop
40.1 MMES-NaOH1reservoirpH6.5
50.2 Mmagnesium acetate1reservoir
620 %(w/v)PEG80001reservoir

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.542 Å
DetectorType: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 14, 2001 / Details: mirrors
RadiationMonochromator: osmic / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.542 Å / Relative weight: 1
ReflectionResolution: 2.1→20 Å / Num. all: 11946 / Num. obs: 11946 / % possible obs: 97.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.6 % / Rmerge(I) obs: 0.099
Reflection shellResolution: 2.1→2.13 Å / Rmerge(I) obs: 0.342 / % possible all: 97.3
Reflection
*PLUS
Lowest resolution: 20 Å / Redundancy: 8.8 %
Reflection shell
*PLUS
Highest resolution: 2.1 Å / % possible obs: 97.3 %

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Processing

Software
NameVersionClassification
MOSFLMdata reduction
SCALAdata scaling
SHELXSphasing
CCP4model building
CNS1.1refinement
CCP4(SCALA)data scaling
CCP4phasing
RefinementMethod to determine structure: MIR / Resolution: 2.1→20 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
RfactorNum. reflection% reflectionSelection details
Rfree0.2666 611 -RANDOM
Rwork0.2207 ---
all0.227 11880 --
obs0.227 11880 96.7 %-
Refinement stepCycle: LAST / Resolution: 2.1→20 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1148 0 0 130 1278
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_bond_d0.010484
X-RAY DIFFRACTIONc_angle_deg1.30799
Refinement
*PLUS
Lowest resolution: 20 Å / Rfactor Rfree: 0.266 / Rfactor Rwork: 0.22
Solvent computation
*PLUS
Displacement parameters
*PLUS
Refine LS restraints
*PLUS
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_bond_d0.01
X-RAY DIFFRACTIONc_angle_deg1.3

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