+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1lvr | ||||||
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タイトル | IC3 of CB1 (L431A,A432L) Bound to G(alpha)i | ||||||
要素 | Cannabinoid receptor 1 | ||||||
キーワード | SIGNALING PROTEIN / intracellular loop 3 (IC3) / cannabinoid 1 receptor (CB1) / alpha domain of G protein i / transferred NOEs | ||||||
機能・相同性 | 機能・相同性情報 cannabinoid signaling pathway / regulation of penile erection / retrograde trans-synaptic signaling by endocannabinoid / cannabinoid receptor activity / negative regulation of mast cell activation / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / negative regulation of fatty acid beta-oxidation / negative regulation of dopamine secretion / positive regulation of acute inflammatory response to antigenic stimulus / regulation of feeding behavior ...cannabinoid signaling pathway / regulation of penile erection / retrograde trans-synaptic signaling by endocannabinoid / cannabinoid receptor activity / negative regulation of mast cell activation / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / negative regulation of fatty acid beta-oxidation / negative regulation of dopamine secretion / positive regulation of acute inflammatory response to antigenic stimulus / regulation of feeding behavior / negative regulation of serotonin secretion / regulation of presynaptic cytosolic calcium ion concentration / negative regulation of action potential / Class A/1 (Rhodopsin-like receptors) / positive regulation of blood pressure / positive regulation of fever generation / regulation of metabolic process / axonal fasciculation / regulation of synaptic transmission, GABAergic / regulation of insulin secretion / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / GABA-ergic synapse / maternal process involved in female pregnancy / regulation of synaptic transmission, glutamatergic / negative regulation of blood pressure / response to nutrient / response to cocaine / G protein-coupled receptor activity / response to nicotine / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / adenylate cyclase-activating G protein-coupled receptor signaling pathway / memory / positive regulation of neuron projection development / actin cytoskeleton / glucose homeostasis / presynaptic membrane / G alpha (i) signalling events / growth cone / spermatogenesis / response to ethanol / mitochondrial outer membrane / response to lipopolysaccharide / positive regulation of apoptotic process / membrane raft / glutamatergic synapse / identical protein binding / plasma membrane / cytoplasm 類似検索 - 分子機能 | ||||||
手法 | 溶液NMR / metric matrix distance geometry to generate initial ensemble of structures; 20 lowest penalty structures used for ensemble-based IRMA refinement | ||||||
データ登録者 | Ulfers, A.L. / McMurry, J.L. / Miller, A. / Wang, L. / Kendall, D.A. / Mierke, D.F. | ||||||
引用 | ジャーナル: Protein Sci. / 年: 2002 タイトル: Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity. 著者: Ulfers, A.L. / McMurry, J.L. / Miller, A. / Wang, L. / Kendall, D.A. / Mierke, D.F. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1lvr.cif.gz | 9.5 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1lvr.ent.gz | 5.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1lvr.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1lvr_validation.pdf.gz | 236.8 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1lvr_full_validation.pdf.gz | 236.6 KB | 表示 | |
XML形式データ | 1lvr_validation.xml.gz | 1.3 KB | 表示 | |
CIF形式データ | 1lvr_validation.cif.gz | 1.2 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/lv/1lvr ftp://data.pdbj.org/pub/pdb/validation_reports/lv/1lvr | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 1030.262 Da / 分子数: 1 / 断片: IC3 of CB1(residues 338-346) / 変異: L4A,A5L / 由来タイプ: 合成 詳細: The protein was synthesized using solid phase synthesis. The sequence of the protein is naturally found in Homo sapiens. 参照: UniProt: P21554 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: transferred NOEs, build up rates from 6 mixing times |
-試料調製
詳細 | 内容: ALKT 4.0 mM G(alpha)i 200 uM / 溶媒系: acetate buffer, 10 mM |
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試料状態 | イオン強度: 10 mM acetate buffer / pH: 6.0 / 圧: 1 atm / 温度: 308 K |
結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M |
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放射波長 | 相対比: 1 |
NMRスペクトロメーター | タイプ: Bruker AVANCE / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 600 MHz |
-解析
NMR software |
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精密化 | 手法: metric matrix distance geometry to generate initial ensemble of structures; 20 lowest penalty structures used for ensemble-based IRMA refinement ソフトェア番号: 1 | ||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: back calculated data agree with experimental NOESY spectrum 計算したコンフォーマーの数: 20 / 登録したコンフォーマーの数: 1 |