+Open data
-Basic information
Entry | Database: PDB / ID: 1lvr | ||||||
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Title | IC3 of CB1 (L431A,A432L) Bound to G(alpha)i | ||||||
Components | Cannabinoid receptor 1 | ||||||
Keywords | SIGNALING PROTEIN / intracellular loop 3 (IC3) / cannabinoid 1 receptor (CB1) / alpha domain of G protein i / transferred NOEs | ||||||
Function / homology | Function and homology information cannabinoid signaling pathway / regulation of penile erection / retrograde trans-synaptic signaling by endocannabinoid / cannabinoid receptor activity / negative regulation of mast cell activation / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / negative regulation of fatty acid beta-oxidation / negative regulation of dopamine secretion / positive regulation of acute inflammatory response to antigenic stimulus / regulation of feeding behavior ...cannabinoid signaling pathway / regulation of penile erection / retrograde trans-synaptic signaling by endocannabinoid / cannabinoid receptor activity / negative regulation of mast cell activation / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / negative regulation of fatty acid beta-oxidation / negative regulation of dopamine secretion / positive regulation of acute inflammatory response to antigenic stimulus / regulation of feeding behavior / negative regulation of serotonin secretion / regulation of presynaptic cytosolic calcium ion concentration / negative regulation of action potential / Class A/1 (Rhodopsin-like receptors) / positive regulation of blood pressure / positive regulation of fever generation / regulation of metabolic process / axonal fasciculation / regulation of synaptic transmission, GABAergic / regulation of insulin secretion / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / GABA-ergic synapse / maternal process involved in female pregnancy / regulation of synaptic transmission, glutamatergic / negative regulation of blood pressure / response to nutrient / response to cocaine / G protein-coupled receptor activity / response to nicotine / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / adenylate cyclase-activating G protein-coupled receptor signaling pathway / memory / positive regulation of neuron projection development / actin cytoskeleton / glucose homeostasis / presynaptic membrane / G alpha (i) signalling events / growth cone / spermatogenesis / response to ethanol / mitochondrial outer membrane / response to lipopolysaccharide / positive regulation of apoptotic process / membrane raft / glutamatergic synapse / identical protein binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Method | SOLUTION NMR / metric matrix distance geometry to generate initial ensemble of structures; 20 lowest penalty structures used for ensemble-based IRMA refinement | ||||||
Authors | Ulfers, A.L. / McMurry, J.L. / Miller, A. / Wang, L. / Kendall, D.A. / Mierke, D.F. | ||||||
Citation | Journal: Protein Sci. / Year: 2002 Title: Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity. Authors: Ulfers, A.L. / McMurry, J.L. / Miller, A. / Wang, L. / Kendall, D.A. / Mierke, D.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1lvr.cif.gz | 9.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1lvr.ent.gz | 5.3 KB | Display | PDB format |
PDBx/mmJSON format | 1lvr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1lvr_validation.pdf.gz | 236.8 KB | Display | wwPDB validaton report |
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Full document | 1lvr_full_validation.pdf.gz | 236.6 KB | Display | |
Data in XML | 1lvr_validation.xml.gz | 1.3 KB | Display | |
Data in CIF | 1lvr_validation.cif.gz | 1.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lv/1lvr ftp://data.pdbj.org/pub/pdb/validation_reports/lv/1lvr | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1030.262 Da / Num. of mol.: 1 / Fragment: IC3 of CB1(residues 338-346) / Mutation: L4A,A5L / Source method: obtained synthetically Details: The protein was synthesized using solid phase synthesis. The sequence of the protein is naturally found in Homo sapiens. References: UniProt: P21554 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: transferred NOEs, build up rates from 6 mixing times |
-Sample preparation
Details | Contents: ALKT 4.0 mM G(alpha)i 200 uM / Solvent system: acetate buffer, 10 mM |
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Sample conditions | Ionic strength: 10 mM acetate buffer / pH: 6.0 / Pressure: 1 atm / Temperature: 308 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: metric matrix distance geometry to generate initial ensemble of structures; 20 lowest penalty structures used for ensemble-based IRMA refinement Software ordinal: 1 | ||||||||||||||||
NMR ensemble | Conformer selection criteria: back calculated data agree with experimental NOESY spectrum Conformers calculated total number: 20 / Conformers submitted total number: 1 |