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- PDB-1ltl: THE DODECAMER STRUCTURE OF MCM FROM ARCHAEAL M. THERMOAUTOTROPHICUM -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ltl | ||||||
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Title | THE DODECAMER STRUCTURE OF MCM FROM ARCHAEAL M. THERMOAUTOTROPHICUM | ||||||
![]() | DNA replication initiator (Cdc21/Cdc54) | ||||||
![]() | REPLICATION | ||||||
Function / homology | ![]() single-stranded 3'-5' DNA helicase activity / MCM complex / double-strand break repair via break-induced replication / DNA unwinding involved in DNA replication / DNA replication initiation / single-stranded DNA binding / DNA helicase / hydrolase activity / ATP binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Fletcher, R.J. / Bishop, B.E. / Leon, R.P. / Sclafani, R.A. / Ogata, C.M. / Chen, X.S. | ||||||
![]() | ![]() Title: The Structure and function of MCM from archaeal M. Thermoautotrophicum Authors: Fletcher, R.J. / Bishop, B.E. / Leon, R.P. / Sclafani, R.A. / Ogata, C.M. / Chen, X.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 292.8 KB | Display | ![]() |
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PDB format | ![]() | 239.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 453.3 KB | Display | ![]() |
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Full document | ![]() | 506.1 KB | Display | |
Data in XML | ![]() | 35.7 KB | Display | |
Data in CIF | ![]() | 52.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32336.662 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid: PGEX-2T / Production host: ![]() ![]() #2: Chemical | ChemComp-ZN / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.35 Å3/Da / Density % sol: 63.3 % | ||||||||||||||||||||||||||||||
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Crystal grow | Details: Hanging drop | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: SBC-2 / Detector: CCD / Date: Dec 8, 2001 |
Radiation | Monochromator: DOUBLE CRYSTAL SI-111, DOUBLE CRYSTAL SI-220 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 3→49.83 Å / Num. obs: 58151 / Observed criterion σ(I): -3 |
Reflection | *PLUS Highest resolution: 3 Å / Lowest resolution: 50 Å / Num. obs: 56524 / Num. measured all: 835966 / Rmerge(I) obs: 99.9 / Rmerge F obs: 0.095 |
Reflection shell | *PLUS % possible obs: 100 % / Rmerge(I) obs: 0.47 |
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Processing
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Refinement | Method to determine structure: ![]() ![]()
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Refinement step | Cycle: LAST / Resolution: 3→20 Å
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Refinement | *PLUS Highest resolution: 3 Å / Lowest resolution: 25 Å / % reflection Rfree: 10 % | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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