+
Open data
-
Basic information
Entry | Database: PDB / ID: 1lpy | ||||||
---|---|---|---|---|---|---|---|
Title | Multiple Methionine Substitutions in T4 Lysozyme | ||||||
![]() | LYSOZYME | ||||||
![]() | HYDROLASE / hydrolase (o-glycosyl) / T4 lysozyme / methionine core mutant / protein engineering / protein folding | ||||||
Function / homology | ![]() viral release from host cell by cytolysis / peptidoglycan catabolic process / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / host cell cytoplasm / defense response to bacterium Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gassner, N.C. / Baase, W.A. / Mooers, B.H.M. / Busam, R.D. / Weaver, L.H. / Lindstrom, J.D. / Quillin, M.L. / Matthews, B.W. | ||||||
![]() | ![]() Title: Multiple methionine substitutions are tolerated in T4 lysozyme and have coupled effects on folding and stability. Authors: Gassner, N.C. / Baase, W.A. / Mooers, B.H. / Busam, R.D. / Weaver, L.H. / Lindstrom, J.D. / Quillin, M.L. / Matthews, B.W. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 49.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 35.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 446.8 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 457.9 KB | Display | |
Data in XML | ![]() | 11.2 KB | Display | |
Data in CIF | ![]() | 15.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1ks3C ![]() 1kw5C ![]() 1kw7C ![]() 1ky0C ![]() 1ky1C ![]() 1l0jC ![]() 1l0kC ![]() 1lw9C ![]() 1lwgC ![]() 1lwkC C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 19654.395 Da / Num. of mol.: 1 Mutation: C54T,L84M,V87M,L91M,C97A,L99M,I100M,V103M,G110R,V111M,L118M,L121M,L133M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|---|
#2: Chemical | ChemComp-PO4 / |
#3: Chemical | ChemComp-CL / |
#4: Chemical | ChemComp-BME / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.46 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS Method: vapor diffusion / Details: Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. / PH range low: 7.1 / PH range high: 6.3 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.65→15 Å / Num. all: 23682 / Num. obs: 23682 / % possible obs: 95 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.043 / Net I/σ(I): 7.1 |
Reflection shell | Resolution: 1.65→1.74 Å / Rmerge(I) obs: 0.103 / Mean I/σ(I) obs: 6.4 / Num. unique all: 1878 |
Reflection | *PLUS Highest resolution: 1.7 Å / Lowest resolution: 15 Å / % possible obs: 96 % |
-
Processing
Software |
| ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: Native T4 Lysozyme Resolution: 1.65→15 Å / σ(F): 0 / Stereochemistry target values: TNT Details: Residues ASN 163 and LEU 164 are missing in the electron density.
| ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.65→15 Å
| ||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.7 Å / Lowest resolution: 15 Å / Rfactor all: 0.209 / Rfactor obs: 0.207 | ||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||
Refine LS restraints | *PLUS Type: t_bond_d / Dev ideal: 0.017 |