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Open data
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Basic information
Entry | Database: PDB / ID: 1lgy | ||||||
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Title | LIPASE II FROM RHIZOPUS NIVEUS | ||||||
![]() | TRIACYLGLYCEROL LIPASE | ||||||
![]() | HYDROLASE (CARBOXYLIC ESTER) / LIPASE | ||||||
Function / homology | ![]() triacylglycerol lipase / triacylglycerol lipase activity / lipid catabolic process / extracellular space / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Kohno, M. / Funatsu, J. / Mikami, B. / Kugimiya, W. / Matsuo, T. / Morita, Y. | ||||||
![]() | ![]() Title: The crystal structure of lipase II from Rhizopus niveus at 2.2 A resolution. Authors: Kohno, M. / Funatsu, J. / Mikami, B. / Kugimiya, W. / Matsuo, T. / Morita, Y. #1: ![]() Title: Purification, Characterization and Crystallization of Two Types of Lipase from Rhizopus Niveus Authors: Kohno, M. / Kugimiya, W. / Hashimoto, Y. / Morita, Y. #2: ![]() Title: Preliminary Investigation of Crystals of Lipase I from Rhizopus Niveus Authors: Kohno, M. / Kugimiya, W. / Hashimoto, Y. / Morita, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 171.1 KB | Display | ![]() |
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PDB format | ![]() | 136.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 380.6 KB | Display | ![]() |
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Full document | ![]() | 403.6 KB | Display | |
Data in XML | ![]() | 18.8 KB | Display | |
Data in CIF | ![]() | 30.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 29623.504 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 47 % | ||||||||||||||||||||
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Crystal | *PLUS | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 6.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Jan 16, 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 53656 / % possible obs: 86.7 % / Rmerge(I) obs: 0.089 |
Reflection | *PLUS Highest resolution: 2 Å / % possible obs: 78 % |
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Processing
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Refinement | Resolution: 2.2→10 Å / σ(F): 2 Details: CHARMM MEAN B VALUE 22.6 ANGSTROMS**2 ESD FROM LUZZATI PLOT 0.30 ANGSTROMS X-PLOR ALSO WAS USED.
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Refinement step | Cycle: LAST / Resolution: 2.2→10 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.186 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |