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Open data
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Basic information
Entry | Database: PDB / ID: 1l6u | |||||||||
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Title | NMR STRUCTURE OF OXIDIZED ADRENODOXIN | |||||||||
![]() | Adrenodoxin 1 | |||||||||
![]() | ELECTRON TRANSPORT / [2Fe-2S]-cluster / primary interaction domain (helix from Asp72-Asp79) / (alpha-beta)-Protein / 5 Helices / 5 beta strands | |||||||||
Function / homology | ![]() Mitochondrial iron-sulfur cluster biogenesis / Pregnenolone biosynthesis / Endogenous sterols / Electron transport from NADPH to Ferredoxin / hormone biosynthetic process / P450-containing electron transport chain / steroid biosynthetic process / respiratory electron transport chain / cholesterol metabolic process / electron transport chain ...Mitochondrial iron-sulfur cluster biogenesis / Pregnenolone biosynthesis / Endogenous sterols / Electron transport from NADPH to Ferredoxin / hormone biosynthetic process / P450-containing electron transport chain / steroid biosynthetic process / respiratory electron transport chain / cholesterol metabolic process / electron transport chain / 2 iron, 2 sulfur cluster binding / electron transfer activity / mitochondrial matrix / protein homodimerization activity / mitochondrion / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | SOLUTION NMR / distance geometry, simulated annealing | |||||||||
![]() | Beilke, D. / Weiss, R. / Lohr, F. / Pristovsek, P. / Hannemann, F. / Bernhardt, R. / Rueterjans, H. | |||||||||
![]() | ![]() Title: A new electron transport mechanism in mitochondrial steroid hydroxylase systems based on structural changes upon the reduction of adrenodoxin. Authors: Beilke, D. / Weiss, R. / Lohr, F. / Pristovsek, P. / Hannemann, F. / Bernhardt, R. / Ruterjans, H. #1: ![]() Title: NEW ASPECTS OF ELECTRON TRANSFER REVEALED BY THE CRYSTAL STRUCTURE OF A TRUNCATED BOVINE ADRENODOXIN, ADX(4-108) Authors: Muller, A. / Muller, J.J. / Muller, Y.A. / Uhlmann, H. / Bernhardt, R. / Heinemann, U. | |||||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 329.4 KB | Display | ![]() |
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PDB format | ![]() | 279.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 433.7 KB | Display | ![]() |
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Full document | ![]() | 551.2 KB | Display | |
Data in XML | ![]() | 32.8 KB | Display | |
Data in CIF | ![]() | 42.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1l6vC C: citing same article ( |
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Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: Protein | Mass: 14061.761 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-FES / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy |
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Sample preparation
Details | Contents: 2-3mM Adrenodoxin 15N,13C, 50 mM phosphate buffer, 50mM NaCl, 10% D2O, 0.04% NaN3 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 100 mM salt / pH: 7.4 / Pressure: 1 atm / Temperature: 300 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: distance geometry, simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 10 |