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- PDB-1kgw: THREE DIMENSIONAL STRUCTURE ANALYSIS OF THE R337Q VARIANT OF HUMA... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1kgw | |||||||||
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Title | THREE DIMENSIONAL STRUCTURE ANALYSIS OF THE R337Q VARIANT OF HUMAN PANCREATIC ALPHA-MYLASE | |||||||||
![]() | ALPHA-AMYLASE, PANCREATIC | |||||||||
![]() | HYDROLASE / ALPHA-AMYLASE / CHLORIDE BINDING / PICHIA PASTORIS / MUTAGENESIS / CATALYSIS / PANCREATIC / ENZYME / HUMAN | |||||||||
Function / homology | ![]() polysaccharide digestion / Digestion of dietary carbohydrate / alpha-amylase / Developmental Lineage of Pancreatic Acinar Cells / alpha-amylase activity / carbohydrate catabolic process / chloride ion binding / carbohydrate metabolic process / calcium ion binding / extracellular space ...polysaccharide digestion / Digestion of dietary carbohydrate / alpha-amylase / Developmental Lineage of Pancreatic Acinar Cells / alpha-amylase activity / carbohydrate catabolic process / chloride ion binding / carbohydrate metabolic process / calcium ion binding / extracellular space / extracellular exosome / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Numao, S. / Maurus, R. / Sidhu, G. / Wang, Y. / Overall, C.M. / Brayer, G.D. / Withers, S.G. | |||||||||
![]() | ![]() Title: Probing the role of the chloride ion in the mechanism of human pancreatic alpha-amylase. Authors: Numao, S. / Maurus, R. / Sidhu, G. / Wang, Y. / Overall, C.M. / Brayer, G.D. / Withers, S.G. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 114.7 KB | Display | ![]() |
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PDB format | ![]() | 86.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 395.1 KB | Display | ![]() |
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Full document | ![]() | 402.4 KB | Display | |
Data in XML | ![]() | 12 KB | Display | |
Data in CIF | ![]() | 18.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1kb3C ![]() 1kguC ![]() 1kgxC ![]() 1bsiS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 55902.238 Da / Num. of mol.: 1 / Mutation: R377Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Sugar | ChemComp-NAG / |
#3: Chemical | ChemComp-CA / |
#4: Water | ChemComp-HOH / |
Has protein modification | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 49.39 % | ||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 / Details: Brayer, G.D., (2000) Biochemistry, 39, 4778. | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Jul 15, 1999 / Details: MIRRORS |
Radiation | Monochromator: MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→30 Å / Num. all: 29654 / Num. obs: 29654 / Observed criterion σ(I): 0 / Redundancy: 3.8 % / Rmerge(I) obs: 0.056 / Net I/σ(I): 17.3 |
Reflection | *PLUS Highest resolution: 2 Å / Lowest resolution: 30 Å / Redundancy: 3.8 % / Num. measured all: 297678 |
Reflection shell | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 2.14 Å / Redundancy: 2.2 % / Rmerge(I) obs: 0.134 / Mean I/σ(I) obs: 8.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1BSI Resolution: 2.1→10 Å / Isotropic thermal model: ISOTROPIC / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 22.6 Å2 | ||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.23 Å | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→10 Å
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Software | *PLUS Name: ![]() | ||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.19 | ||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||
Refine LS restraints | *PLUS
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