+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1jm4 | |||||||||
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タイトル | NMR Structure of P/CAF Bromodomain in Complex with HIV-1 Tat Peptide | |||||||||
要素 |
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キーワード | TRANSFERASE / Bromodomain / Protein-peptide Complex | |||||||||
機能・相同性 | 機能・相同性情報 viral gene expression / trans-activation response element binding / regulation of protein ADP-ribosylation / regulatory region RNA binding / negative regulation of cellular respiration / protein serine/threonine phosphatase inhibitor activity / negative regulation of rRNA processing / positive regulation of viral transcription / histone H3K9 acetyltransferase activity / diamine N-acetyltransferase ...viral gene expression / trans-activation response element binding / regulation of protein ADP-ribosylation / regulatory region RNA binding / negative regulation of cellular respiration / protein serine/threonine phosphatase inhibitor activity / negative regulation of rRNA processing / positive regulation of viral transcription / histone H3K9 acetyltransferase activity / diamine N-acetyltransferase / positive regulation of transcription from RNA polymerase II promoter by glucose / diamine N-acetyltransferase activity / negative regulation of centriole replication / positive regulation of attachment of mitotic spindle microtubules to kinetochore / Physiological factors / peptidyl-lysine acetylation / lysine N-acetyltransferase activity, acting on acetyl phosphate as donor / symbiont-mediated perturbation of host chromatin organization / symbiont-mediated suppression of host translation initiation / YAP1- and WWTR1 (TAZ)-stimulated gene expression / positive regulation of fatty acid biosynthetic process / actomyosin / internal peptidyl-lysine acetylation / histone H3 acetyltransferase activity / host cell nucleolus / cyclin-dependent protein serine/threonine kinase inhibitor activity / membrane hyperpolarization / N-terminal peptidyl-lysine acetylation / ATAC complex / actinin binding / I band / cellular response to parathyroid hormone stimulus / SAGA complex / Regulation of FOXO transcriptional activity by acetylation / limb development / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RUNX3 regulates NOTCH signaling / A band / NOTCH4 Intracellular Domain Regulates Transcription / NOTCH3 Intracellular Domain Regulates Transcription / peptide-lysine-N-acetyltransferase activity / regulation of tubulin deacetylation / histone acetyltransferase binding / protein acetylation / Formation of WDR5-containing histone-modifying complexes / Notch-HLH transcription pathway / negative regulation of peptidyl-threonine phosphorylation / Formation of paraxial mesoderm / acetyltransferase activity / regulation of cell division / RNA Polymerase I Transcription Initiation / regulation of RNA splicing / regulation of embryonic development / RNA-binding transcription regulator activity / regulation of DNA repair / histone acetyltransferase activity / histone acetyltransferase / positive regulation of gluconeogenesis / positive regulation of glycolytic process / cyclin binding / transcription initiation-coupled chromatin remodeling / gluconeogenesis / RNA polymerase II transcription regulatory region sequence-specific DNA binding / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / B-WICH complex positively regulates rRNA expression / mitotic spindle / Metalloprotease DUBs / NOTCH1 Intracellular Domain Regulates Transcription / kinetochore / memory / positive regulation of neuron projection development / Pre-NOTCH Transcription and Translation / histone deacetylase binding / cellular response to insulin stimulus / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / positive regulation of protein localization to nucleus / vasodilation / rhythmic process / positive regulation of NF-kappaB transcription factor activity / heart development / HATs acetylate histones / cellular response to oxidative stress / DNA-binding transcription factor binding / Estrogen-dependent gene expression / host cell cytoplasm / transcription coactivator activity / regulation of cell cycle / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / symbiont-mediated suppression of host innate immune response / chromatin remodeling / protein domain specific binding / negative regulation of cell population proliferation / virus-mediated perturbation of host defense response / centrosome / DNA-templated transcription / chromatin binding / regulation of DNA-templated transcription 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 溶液NMR / Distance Geometry, Simulated Annealing | |||||||||
データ登録者 | Mujtaba, S. / He, Y. / Zeng, L. / Farooq, A. / Carlson, J.E. / Ott, M. / Verdin, E. / Zhou, M.-M. | |||||||||
引用 | ジャーナル: Mol.Cell / 年: 2002 タイトル: Structural basis of lysine-acetylated HIV-1 Tat recognition by PCAF bromodomain 著者: Mujtaba, S. / He, Y. / Zeng, L. / Farooq, A. / Carlson, J.E. / Ott, M. / Verdin, E. / Zhou, M.-M. #1: ジャーナル: Nature / 年: 1999 タイトル: Structure and Ligand of a Histone Acetyltransferase Bromodomain 著者: Dhalluin, C. / Carlson, J.E. / Zeng, L. / He, C. / Aggarwal, A.K. / Zhou, M.-M. | |||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1jm4.cif.gz | 1 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1jm4.ent.gz | 884.4 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1jm4.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1jm4_validation.pdf.gz | 373.8 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1jm4_full_validation.pdf.gz | 684.9 KB | 表示 | |
XML形式データ | 1jm4_validation.xml.gz | 94.4 KB | 表示 | |
CIF形式データ | 1jm4_validation.cif.gz | 116.5 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/jm/1jm4 ftp://data.pdbj.org/pub/pdb/validation_reports/jm/1jm4 | HTTPS FTP |
-関連構造データ
関連構造データ | |
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類似構造データ |
-リンク
-集合体
登録構造単位 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 1484.754 Da / 分子数: 1 / 由来タイプ: 合成 / 詳細: The Peptide was Chemically Synthesized. / 参照: UniProt: P04610*PLUS |
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#2: タンパク質 | 分子量: 14052.226 Da / 分子数: 1 / Fragment: Bromodomain / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / プラスミド: pET14b / 生物種 (発現宿主): Escherichia coli / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21(DE3) / 参照: UniProt: Q92831 |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 |
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試料状態 | イオン強度: 100 mM / pH: 6.5 / 圧: Ambient / 温度: 303 K | ||||||||||||
結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M | |||||||||||||||
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放射波長 | 相対比: 1 | |||||||||||||||
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: Distance Geometry, Simulated Annealing / ソフトェア番号: 1 詳細: THE ARIA/X-PLOR PLATFORM HAS BEEN USED FOR THE NOE ASSIGNMENT | ||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: Back Calculated Data Agree with Experimental NOESY Spectrum, Structures with Acceptable Covalent Geometry, Structures with Favorable Non-bond Energy, Structures ...コンフォーマー選択の基準: Back Calculated Data Agree with Experimental NOESY Spectrum, Structures with Acceptable Covalent Geometry, Structures with Favorable Non-bond Energy, Structures with the Least Restraint Violations, Structures with the Lowest Energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 25 |