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- PDB-1je4: Solution structure of the monomeric variant of the chemokine MIP-1beta -
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Open data
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Basic information
Entry | Database: PDB / ID: 1je4 | ||||||
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Title | Solution structure of the monomeric variant of the chemokine MIP-1beta | ||||||
![]() | macrophage inflammatory protein 1-beta | ||||||
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Function / homology | ![]() CCR1 chemokine receptor binding / positive regulation of natural killer cell chemotaxis / CCR5 chemokine receptor binding / CCR chemokine receptor binding / lymphocyte chemotaxis / positive regulation of calcium ion transport / eosinophil chemotaxis / chemokine-mediated signaling pathway / Chemokine receptors bind chemokines / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
Model type details | minimized average | ||||||
![]() | Kim, S. / Jao, S. / Laurence, J.S. / LiWang, P.J. | ||||||
![]() | ![]() Title: Structural comparison of monomeric variants of the chemokine MIP-1beta having differing ability to bind the receptor CCR5. Authors: Kim, S. / Jao, S. / Laurence, J.S. / LiWang, P.J. #1: ![]() Title: CC chemokine MIP-1beta can function as a monomer and depends on Phe13 for receptor binding Authors: Laurence, J.S. / Blanpain, C. / Burgner, J.W. / Parmentier, M. / LiWang, P.J. #2: ![]() Title: High-resolution solution structure of the beta chemokine hMIP-1beta by multidimensional NMR Authors: Lodi, P.J. / Garrett, D.S. / Kuszewski, J. / Tsang, M.L. / Weatherbee, J.A. / Leonard, W.J. / Gronenborn, A.M. / Clore, G.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 30.2 KB | Display | ![]() |
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PDB format | ![]() | 23.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 7748.646 Da / Num. of mol.: 1 / Mutation: F13A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 3D 15N or 13C edited NMR experiments. |
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Sample preparation
Details | Contents: 1-2mM MIP-1b F13A U-15N, 13C; 20mM Na-phosphate buffer Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 20mM sodium phosphate / pH: 2.5 / Pressure: ambient / Temperature: 298 K |
Crystal grow![]() | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: The initial fold was obtained by distance geometry, further refined by simulated annealing. Software ordinal: 1 Details: The structure is based on a total 940 restraints, 851 distance constraints, 69 dihedral angle restraints, 20 distance restraints for 10 hydrogen bonds. | ||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |