+
Open data
-
Basic information
Entry | Database: PDB / ID: 1ixh | ||||||
---|---|---|---|---|---|---|---|
Title | PHOSPHATE-BINDING PROTEIN (PBP) COMPLEXED WITH PHOSPHATE | ||||||
![]() | PHOSPHATE-BINDING PROTEIN | ||||||
![]() | PHOSPHATE TRANSPORT / ULTRA HIGH RESOLUTION / PHOSPHATE BINDIN PROTEIN | ||||||
Function / homology | ![]() regulation of phosphatase activity / phosphate ion transport / phosphate ion transmembrane transport / phosphate ion binding / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / response to radiation / outer membrane-bounded periplasmic space / DNA damage response / membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wang, Z. / Luecke, H. / Quiocho, F.A. | ||||||
![]() | ![]() Title: A low energy short hydrogen bond in very high resolution structures of protein receptor--phosphate complexes. Authors: Wang, Z. / Luecke, H. / Yao, N. / Quiocho, F.A. #1: ![]() Title: Negative Electrostatic Surface Potential of Protein Sites Specific for Anionic Ligands Authors: Ledvina, P.S. / Yao, N. / Choudhary, A. / Quiocho, F.A. #2: ![]() Title: Modulation of a Salt Link Does not Affect Binding of Phosphate to its Specific Active Transport Receptor Authors: Yao, N. / Ledvina, P.S. / Choudhary, A. / Quiocho, F.A. #3: ![]() Title: Fine Tuning the Specificity of the Periplasmic Phosphate Transport Receptor. Site-Directed Mutagenesis, Ligand Binding, and Crystallographic Studies Authors: Wang, Z. / Choudhary, A. / Ledvina, P.S. / Quiocho, F.A. #4: ![]() Title: The Immunodominant 38-kDa Lipoprotein Antigen of Mycobacterium Tuberculosis is a Phosphate-Binding Protein Authors: Chang, Z. / Choudhary, A. / Lathigra, R. / Quiocho, F.A. #5: ![]() Title: High Specificity of a Phosphate Transport Protein Determined by Hydrogen Bonds Authors: Luecke, H. / Quiocho, F.A. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 208.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 165 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 365.2 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 368.8 KB | Display | |
Data in XML | ![]() | 7.8 KB | Display | |
Data in CIF | ![]() | 11.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1ixgC ![]() 1ixiC ![]() 1abh S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 34457.598 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|---|
#2: Chemical | ChemComp-PO4 / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 40 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | pH: 4.5 / Details: pH 4.5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / Details: Wang, Z., (1994) J.Biol.Chem., 269, 25091. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: IMAGE PLATE / Date: Jan 1, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 0.98→19.4 Å / Num. obs: 147105 / % possible obs: 79.8 % / Observed criterion σ(I): 0 / Redundancy: 9.4 % / Rmerge(I) obs: 0.053 / Net I/σ(I): 35.3 |
Reflection shell | Resolution: 0.98→1 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.246 / Mean I/σ(I) obs: 3.6 / % possible all: 29.8 |
-
Processing
Software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1ABH ![]() 1abh Resolution: 0.98→19.4 Å / Num. parameters: 28413 / Num. restraintsaints: 0 / Cross valid method: FREE R / σ(F): 0
| ||||||||||||||||
Solvent computation | Solvent model: MOEWS & KRETSINGER | ||||||||||||||||
Refine analyze | Num. disordered residues: 6 / Occupancy sum hydrogen: 547 / Occupancy sum non hydrogen: 3110 | ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 0.98→19.4 Å
|