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- PDB-1i4h: Crystal structure of Zn2+ soaked Staphylococcal enterotoxin A mut... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1i4h | ||||||
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Title | Crystal structure of Zn2+ soaked Staphylococcal enterotoxin A mutant H187A | ||||||
![]() | ENTEROTOXIN TYPE A | ||||||
![]() | TOXIN / beta-grasp / beta-barrel | ||||||
Function / homology | ![]() MHC class II protein binding / toxin activity / extracellular region / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Hakansson, M. / Antonsson, P. / Bjork, P. / Svensson, L.A. | ||||||
![]() | ![]() Title: Cooperative zinc binding in a staphylococcal enterotoxin A mutant mimics the SEA-MHC class II interaction Authors: Hakansson, M. / Antonsson, P. / Bjork, P. / Svensson, L.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 93.7 KB | Display | ![]() |
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PDB format | ![]() | 73.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1i4gSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27063.236 Da / Num. of mol.: 2 / Mutation: H187A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 46 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: evaporation / pH: 6 Details: PEG 8000, lithium sulphate, pipes, zinc chloride, pH 6.0, EVAPORATION, temperature 298K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 8 / Method: vapor diffusionDetails: drop was mixed with an equal volume of reservoir solution | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 19, 1996 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→20 Å / Num. all: 10658 / Num. obs: 10658 / % possible obs: 84.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.1 % / Biso Wilson estimate: 58.9 Å2 / Rmerge(I) obs: 0.087 / Net I/σ(I): 13.2 |
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 4 % / Rmerge(I) obs: 0.269 / Mean I/σ(I) obs: 3.2 / Num. unique all: 1039 / % possible all: 88.1 |
Reflection | *PLUS Num. measured all: 43647 |
Reflection shell | *PLUS % possible obs: 88.1 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: pdb entry 1I4G Resolution: 2.9→20 Å / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 40.9 Å2 | |||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.9→20 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | |||||||||||||||||||||
Refine LS restraints | *PLUS
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