+Open data
-Basic information
Entry | Database: PDB / ID: 1hrl | ||||||
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Title | STRUCTURE OF A PARALYTIC PEPTIDE FROM AN INSECT, MANDUCA SEXTA | ||||||
Components | PARALYTIC PEPTIDE I | ||||||
Keywords | TOXIN / paralytic peptide / plasmatocyte spreading peptide / ENF family | ||||||
Function / homology | Paralytic/GBP/PSP peptide / Paralytic/GBP/PSP peptide / cytokine activity / extracellular space / Paralytic peptide 1 Function and homology information | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Yu, X.-Q. / Prakash, O. / Kanost, M.R. | ||||||
Citation | Journal: J.Pept.Res. / Year: 1999 Title: Structure of a paralytic peptide from an insect, Manduca sexta. Authors: Yu, X.Q. / Prakash, O. / Kanost, M.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hrl.cif.gz | 62.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hrl.ent.gz | 48.4 KB | Display | PDB format |
PDBx/mmJSON format | 1hrl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1hrl_validation.pdf.gz | 341.7 KB | Display | wwPDB validaton report |
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Full document | 1hrl_full_validation.pdf.gz | 403.7 KB | Display | |
Data in XML | 1hrl_validation.xml.gz | 7.5 KB | Display | |
Data in CIF | 1hrl_validation.cif.gz | 10.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hr/1hrl ftp://data.pdbj.org/pub/pdb/validation_reports/hr/1hrl | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2438.719 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This sequence occurs naturally in the tobacco hornworm, Manduca sexta References: UniProt: P30253 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: The structures were determined using standard 2D homonuclear techniques |
-Sample preparation
Details | Contents: 3 mM in 90% H2O, 10% D2O at pH 4.3 / Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 0 / pH: 4.3 / Pressure: ambient / Temperature: 283 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITYPLUS / Manufacturer: Varian / Model: UNITYPLUS / Field strength: 500 MHz |
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-Processing
NMR software | Name: X-PLOR / Version: 3.1 / Developer: Brunger, A.T. / Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |