- PDB-1hpw: STRUCTURE OF A PILIN MONOMER FROM PSEUDOMONAS AERUGINOSA: IMPLICA... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 1hpw
Title
STRUCTURE OF A PILIN MONOMER FROM PSEUDOMONAS AERUGINOSA: IMPLICATIONS FOR THE ASSEMBLY OF PILI.
Components
FIMBRIAL PROTEIN
Keywords
CONTRACTILE PROTEIN / fimbria / methylation
Function / homology
Function and homology information
glycosphingolipid binding / biological process involved in interaction with host / pilus / periplasmic space / cell adhesion / membrane Similarity search - Function
Mass: 13116.644 Da / Num. of mol.: 1 / Fragment: PILIN (RESIDUES 29-150) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Strain: K122-4 / Plasmid: PRLD / Production host: Escherichia coli (E. coli) / Strain (production host): DH5ALPHA / References: UniProt: P17838
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D NOESY
1
2
1
3D 15N-separated NOESY
1
3
1
DQF-COSY
1
4
1
HNHA
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Sample preparation
Details
Contents: 0.5 mM K122-4 pilin dissolved in either 90% H2O/10% D2O or 99% D2O containing 20 mM deuterated sodium acetate, 1 mM NaN3 and 1 mM DSS, pH 5.0.
Sample conditions
pH: 5.1 / Temperature: 303 K
Crystal grow
*PLUS
Method: other / Details: NMR
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NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Varian UNITY
Varian
UNITY
600
1
Varian INOVA
Varian
INOVA
800
2
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Processing
NMR software
Name
Version
Developer
Classification
VNMR
6.2B
Varian
collection
NMRPipe
Delaglio
processing
NMRView
4.2
Johnson
dataanalysis
X-PLOR
3.851
Brunger
refinement
Refinement
Method: distance geometry simulated annealing / Software ordinal: 1 Details: The structures are based on a total of 1213 restraints, 1032 are NOE-derived distance constraints, 181 dihedral angle restraints, 30 distance restraints from hydrogen bonds.
NMR representative
Selection criteria: closest to the average
NMR ensemble
Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 10
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