estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate ...estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate / feeding behavior / response to growth hormone / response to food / positive regulation of Rho protein signal transduction / positive regulation of peptide hormone secretion / protein secretion / negative regulation of lipid catabolic process / response to glucose / response to nutrient levels / positive regulation of protein secretion / insulin receptor binding / positive regulation of insulin secretion / hormone activity / glucose metabolic process / glucose homeostasis / response to heat / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of gene expression / negative regulation of apoptotic process / extracellular space / identical protein binding Similarity search - Function
Insulin / Insulin family / Insulin/IGF/Relaxin family / Insulin, conserved site / Insulin family signature. / Insulin-like / Insulin / insulin-like growth factor / relaxin family. / Insulin-like superfamily Similarity search - Domain/homology
structures with the least restraint violations, structures with the lowest energy
Representative
Model #21
closest to the average
-
Components
#1: Protein/peptide
INSULIN
Mass: 3371.797 Da / Num. of mol.: 1 / Fragment: B-CHAIN / Mutation: C7S, C19S / Source method: obtained synthetically Details: THIS PEPTIDE WAS CHEMICALLY SYNTHESIZED. IT WAS Obtained by solid phase synthesis using a peptide synthesizer. References: UniProt: P01317
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
DQF-COSY
1
2
1
TOCSY
1
3
1
NOESY
NMR details
Text: This family of structures was determined using standard 2D homonuclear techniques.
Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz
-
Processing
NMR software
Name
Version
Developer
Classification
XwinNMR
2.1
BrukerSA
processing
Gifa
4.2
Delsucetal. (UniversitedeMontpellier, France)
processing
Insight II
98
MolecularSimulationInc.
dataanalysis
NMR-Refine
98
MolecularSimulationInc.
refinement
Refinement
Method: simulated annealing / Software ordinal: 1 Details: These structures are based on a total of 286 restraints: 243 are NOE-derived distance constraints, 39 dihedral angle restraints and 4 distance restraints from hydrogen bonds.
NMR representative
Selection criteria: closest to the average
NMR ensemble
Conformer selection criteria: structures with the least restraint violations, structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 21
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