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Yorodumi- PDB-1h1j: The SAP domain is a DNA-Binding Domain Capable of Binding S/MAR DNA -
+Open data
-Basic information
Entry | Database: PDB / ID: 1h1j | ||||||
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Title | The SAP domain is a DNA-Binding Domain Capable of Binding S/MAR DNA | ||||||
Components | THO1 PROTEIN | ||||||
Keywords | DNA BINDING / SAP DOMAIN | ||||||
Function / homology | Function and homology information poly(A)+ mRNA export from nucleus / protein-RNA complex assembly / transcription elongation by RNA polymerase II / double-stranded DNA binding / chromatin binding / RNA binding / nucleus Similarity search - Function | ||||||
Biological species | SACCHAROMYCES CEREVISIAE (brewer's yeast) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Jacobsen, J.O.B. / Freund, S.M.V. / Bycroft, M. | ||||||
Citation | Journal: To be Published Title: The Sap Domain is a DNA-Binding Domain Capable of Binding S/Mar DNA Authors: Jacobsen, J.O.B. / Freund, S.M.V. / Bycroft, M. #1: Journal: Trends Biochem.Sci. / Year: 2000 Title: Sap - a Putative DNA-Binding Motif Involved in Chromosomal Organization Authors: Aravind, L. / Koonin, E.V. #2: Journal: Embo J. / Year: 1998 Title: A Novel Yeast Gene, Tho2, is Involved in RNA Pol II Transcription and Provides New Evidence for Transcriptional Elongation-Associated Recombination. Authors: Piruat, J.I. / Aguilera, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1h1j.cif.gz | 267.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1h1j.ent.gz | 225.9 KB | Display | PDB format |
PDBx/mmJSON format | 1h1j.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1h1j_validation.pdf.gz | 343.8 KB | Display | wwPDB validaton report |
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Full document | 1h1j_full_validation.pdf.gz | 509.5 KB | Display | |
Data in XML | 1h1j_validation.xml.gz | 36.8 KB | Display | |
Data in CIF | 1h1j_validation.cif.gz | 54.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h1/1h1j ftp://data.pdbj.org/pub/pdb/validation_reports/h1/1h1j | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5582.212 Da / Num. of mol.: 1 / Fragment: SAP DOMAIN, RESIDUES 2-50 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Plasmid: PGRO / Production host: ESCHERICHIA COLI BL21(DE3) (bacteria) / Variant (production host): C41 / References: UniProt: P40040 |
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Sequence details | GLY 1, THROMBIN CLEAVAGE SITE INSERTION SER 2, THROMBIN CLEAVAGE SITE INSERTION |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: STRUCTURE DETERMINED USING STANDARD TRIPLE-RESONANCE AND 3D 1H,13C- NOESY/TOCSY EXPERIMENTS ON 13C, 15N-LABELED PROTEIN. |
-Sample preparation
Details | Contents: 1.5MM |
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Sample conditions | Ionic strength: 150 mM / pH: 6.8 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. RESIDUES 45-51 ARE DISORDERED WITH NO DEFINED STRUCTURE AND NOT INCLUDED IN THE COORDINATES. | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST NOE ENERGIES / Conformers calculated total number: 50 / Conformers submitted total number: 20 |