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- PDB-1g4w: CRYSTAL STRUCTURE OF THE SALMONELLA TYROSINE PHOSPHATASE AND GTPA... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1g4w | ||||||
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Title | CRYSTAL STRUCTURE OF THE SALMONELLA TYROSINE PHOSPHATASE AND GTPASE ACTIVATING PROTEIN SPTP | ||||||
![]() | PROTEIN TYROSINE PHOSPHATASE SPTP | ||||||
![]() | SIGNALING PROTEIN / virulence factor / tyrosine phosphatase / GTPase activating protein / 4-helix bundle / disorder | ||||||
Function / homology | ![]() dephosphorylation / protein-tyrosine-phosphatase / GTPase activator activity / protein tyrosine phosphatase activity / host cell cytoplasm / extracellular space Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Stebbins, C.E. / Galan, J.E. | ||||||
![]() | ![]() Title: Modulation of host signaling by a bacterial mimic: structure of the Salmonella effector SptP bound to Rac1. Authors: Stebbins, C.E. / Galan, J.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 80.5 KB | Display | ![]() |
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PDB format | ![]() | 60.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 362.7 KB | Display | ![]() |
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Full document | ![]() | 375.1 KB | Display | |
Data in XML | ![]() | 9.4 KB | Display | |
Data in CIF | ![]() | 14.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 42127.801 Da / Num. of mol.: 1 / Fragment: SPTP RESIDUES 161-543 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.99 % | ||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 3.5M sodium formate, 2mM DTT, 0.1M Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||
Crystal | *PLUS Density % sol: 52 % | ||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Sep 21, 1999 / Details: Yale mirrors |
Radiation | Monochromator: Yale Mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. all: 24740 / Num. obs: 23440 / % possible obs: 94.8 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 4.8 % / Biso Wilson estimate: 21.7 Å2 / Rmerge(I) obs: 0.077 / Net I/σ(I): 13.9 |
Reflection shell | Resolution: 2.2→2.3 Å / Redundancy: 2 % / Rmerge(I) obs: 0.337 / Mean I/σ(I) obs: 2.93 / % possible all: 94.9 |
Reflection | *PLUS Highest resolution: 2.2 Å / Num. measured all: 114569 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.2→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.23 Å /
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 50 Å / σ(F): 0 / % reflection Rfree: 7 % / Rfactor obs: 0.26 / Rfactor Rwork: 0.26 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.4 | ||||||||||||||||||||
LS refinement shell | *PLUS Rfactor Rfree: 0.392 / Rfactor Rwork: 0.381 |