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Open data
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Basic information
Entry | Database: PDB / ID: 1g2c | ||||||
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Title | HUMAN RESPIRATORY SYNCYTIAL VIRUS FUSION PROTEIN CORE | ||||||
![]() | (FUSION PROTEIN (F)) x 2 | ||||||
![]() | VIRAL PROTEIN / membrane fusion / pneumovirus / HRSV | ||||||
Function / homology | ![]() positive regulation of syncytium formation by virus / host cell Golgi membrane / membrane => GO:0016020 / entry receptor-mediated virion attachment to host cell / fusion of virus membrane with host plasma membrane / viral envelope / host cell plasma membrane / virion membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Zhao, X. / Singh, M. / Malashkevich, V.N. / Kim, P.S. | ||||||
![]() | ![]() Title: Structural characterization of the human respiratory syncytial virus fusion protein core. Authors: Zhao, X. / Singh, M. / Malashkevich, V.N. / Kim, P.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 221.8 KB | Display | ![]() |
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PDB format | ![]() | 182.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 499.6 KB | Display | ![]() |
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Full document | ![]() | 537.4 KB | Display | |
Data in XML | ![]() | 23.9 KB | Display | |
Data in CIF | ![]() | 42 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Details | The biological assembly is a trimer of heterodimer constructed from chain A, B, C,D,E,F |
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Components
#1: Protein | Mass: 5653.654 Da / Num. of mol.: 12 / Fragment: RESIDUES 153-209, HRSV F1 HEPTAD REPEAT Source method: isolated from a genetically manipulated source Details: FIRST HEPTAD REPEAT / Source: (gene. exp.) ![]() ![]() ![]() #2: Protein/peptide | Mass: 4873.347 Da / Num. of mol.: 12 / Fragment: RESIDUES 476-520, HRSV F1 HEPTAD REPEAT Source method: isolated from a genetically manipulated source Details: SECOND HEPTAD REPEAT / Source: (gene. exp.) ![]() ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 46.9 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: PEG 4000, Tris-HCl, Li2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jul 20, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9816 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→35 Å / Num. all: 62470 / Num. obs: 56973 / % possible obs: 91.2 % / Observed criterion σ(I): -3 / Redundancy: 1.3 % / Biso Wilson estimate: 40.37 Å2 / Rmerge(I) obs: 0.036 / Net I/σ(I): 8.9 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 2 % / Rmerge(I) obs: 0.24 / Num. unique all: 6224 / % possible all: 64.7 |
Reflection | *PLUS Num. measured all: 119905 |
Reflection shell | *PLUS % possible obs: 64.7 % |
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Processing
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Refinement | Starting model: modified polyser SV5 Resolution: 2.3→10 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 1137175.53 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: MLF
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 56.95 Å2 / ksol: 0.283 e/Å3 | ||||||||||||||||||||||||
Displacement parameters | Biso mean: 54.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.3→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.3→2.44 Å / Rfactor Rfree error: 0.012 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.5 / Classification: refinement | ||||||||||||||||||||||||
Refinement | *PLUS σ(F): 0 / % reflection Rfree: 10.2 % | ||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 54.6 Å2 | ||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.352 / % reflection Rfree: 10.1 % / Rfactor Rwork: 0.292 |