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Yorodumi- PDB-1fyc: INNER LIPOYL DOMAIN FROM HUMAN PYRUVATE DEHYDROGENASE (PDH) COMPL... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fyc | ||||||
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Title | INNER LIPOYL DOMAIN FROM HUMAN PYRUVATE DEHYDROGENASE (PDH) COMPLEX, NMR, 1 STRUCTURE | ||||||
Components | DIHYDROLIPOAMIDE ACETYLTRANSFERASE (E2P) | ||||||
Keywords | TRANSFERASE / ACYLTRANSFERASE DIHYDROLIPOAMIDE / SUBUNIT / UNLIPOYLATED | ||||||
Function / homology | Function and homology information dihydrolipoyllysine-residue acetyltransferase / dihydrolipoyllysine-residue acetyltransferase activity / acetyl-CoA biosynthetic process from pyruvate / : / pyruvate dehydrogenase complex / Pyruvate metabolism / Glyoxylate metabolism and glycine degradation / Regulation of pyruvate dehydrogenase (PDH) complex / Signaling by Retinoic Acid / tricarboxylic acid cycle ...dihydrolipoyllysine-residue acetyltransferase / dihydrolipoyllysine-residue acetyltransferase activity / acetyl-CoA biosynthetic process from pyruvate / : / pyruvate dehydrogenase complex / Pyruvate metabolism / Glyoxylate metabolism and glycine degradation / Regulation of pyruvate dehydrogenase (PDH) complex / Signaling by Retinoic Acid / tricarboxylic acid cycle / glucose metabolic process / mitochondrial matrix / intracellular membrane-bounded organelle / mitochondrion / identical protein binding Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Howard, M.J. / Fuller, C. / Broadhurst, R.W. / Quinn, J. / Yeaman, S.J. / Perham, R.N. | ||||||
Citation | Journal: Gastroenterology / Year: 1998 Title: Three-dimensional structure of the major autoantigen in primary biliary cirrhosis. Authors: Howard, M.J. / Fuller, C. / Broadhurst, R.W. / Perham, R.N. / Tang, J.G. / Quinn, J. / Diamond, A.G. / Yeaman, S.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fyc.cif.gz | 43.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fyc.ent.gz | 30.4 KB | Display | PDB format |
PDBx/mmJSON format | 1fyc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fyc_validation.pdf.gz | 340.4 KB | Display | wwPDB validaton report |
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Full document | 1fyc_full_validation.pdf.gz | 356.5 KB | Display | |
Data in XML | 1fyc_validation.xml.gz | 6 KB | Display | |
Data in CIF | 1fyc_validation.cif.gz | 7.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fy/1fyc ftp://data.pdbj.org/pub/pdb/validation_reports/fy/1fyc | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11661.315 Da / Num. of mol.: 1 / Fragment: LIPOYL DOMAIN Source method: isolated from a genetically manipulated source Details: DIHYDROLIPOAMIDE ACETYLTRANSFERASE SUBUNIT OF THE PYRUVATE DEHYDROGENASE (PDH) MULTIENZYME COMPLEX (UNLIPOYLATED DOMAIN) Source: (gene. exp.) Homo sapiens (human) / Description: EXPRESSED AS A GST FUSION PROTEIN / Organ: LIVER / Plasmid: HLIP / Production host: Escherichia coli (E. coli) References: UniProt: P10515, dihydrolipoyllysine-residue acetyltransferase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 6.5 / Temperature: 308 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AM500 / Manufacturer: Bruker / Model: AM500 / Field strength: 500 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAM BE FOUND IN P.M. RICAUD ET AL., JOURNAL OF MOLECULAR BIOLOGY, 264, 179-190, 1996 | ||||||||||||
NMR ensemble | Conformer selection criteria: MEAN STRUCTURE FROM 26 CHOSEN HAVING LEAST RESTRAINT VIOLATION Conformers calculated total number: 80 / Conformers submitted total number: 1 |