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- PDB-1fs8: CYTOCHROME C NITRITE REDUCTASE FROM WOLINELLA SUCCINOGENES-SULFAT... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1fs8 | |||||||||
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Title | CYTOCHROME C NITRITE REDUCTASE FROM WOLINELLA SUCCINOGENES-SULFATE COMPLEX | |||||||||
![]() | CYTOCHROME C NITRITE REDUCTASE | |||||||||
![]() | OXIDOREDUCTASE / c-type cytochrome | |||||||||
Function / homology | ![]() nitrite reductase (cytochrome; ammonia-forming) / nitrite reductase (cytochrome, ammonia-forming) activity / anaerobic electron transport chain / : / nitrate assimilation / outer membrane-bounded periplasmic space / calcium ion binding / heme binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Einsle, O. / Stach, P. / Messerschmidt, A. / Simon, J. / Kroeger, A. / Huber, R. / Kroneck, P.M.H. | |||||||||
![]() | ![]() Title: Cytochrome c nitrite reductase from Wolinella succinogenes. Structure at 1.6 A resolution, inhibitor binding, and heme-packing motifs. Authors: Einsle, O. / Stach, P. / Messerschmidt, A. / Simon, J. / Kroger, A. / Huber, R. / Kroneck, P.M. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 127.9 KB | Display | ![]() |
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PDB format | ![]() | 103.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 747.8 KB | Display | ![]() |
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Full document | ![]() | 757.7 KB | Display | |
Data in XML | ![]() | 12.3 KB | Display | |
Data in CIF | ![]() | 21.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | The biological assembly is a dimer constructed from chain A a symmetry partner generated by the two-fold. |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 55343.602 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Non-polymers , 6 types, 664 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/Y1.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/HEC.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/Y1.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/HEC.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-CA / | ||||||||
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#3: Chemical | ChemComp-Y1 / #4: Chemical | #5: Chemical | ChemComp-ACT / | #6: Chemical | ChemComp-HEC / #7: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.98 Å3/Da / Density % sol: 58.76 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.7 Details: PEG 4000; ammonium sulfate; yttrium trichloride; sodium acetate, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 57 % | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 4, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.05 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→25 Å / Num. all: 88850 / Num. obs: 83904 / % possible obs: 95.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.6 % / Biso Wilson estimate: 23.4 Å2 / Rmerge(I) obs: 0.084 / Net I/σ(I): 11.2 |
Reflection shell | Resolution: 1.6→1.65 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.482 / % possible all: 93.1 |
Reflection shell | *PLUS Mean I/σ(I) obs: 1.5 |
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Processing
Software |
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Refinement | Resolution: 1.6→25 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.6→25 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 25 Å / σ(F): 0 / Rfactor obs: 0.184 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |