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- PDB-1frn: THE INVOLVEMENT OF SER96 IN THE CATALYTIC MECHANISM OF FERREDOXIN... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1frn | ||||||
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Title | THE INVOLVEMENT OF SER96 IN THE CATALYTIC MECHANISM OF FERREDOXIN-NADP+ REDUCTASE: STRUCTURE-FUNCTION RELATIONSHIP AS STUDIED BY SITE-DIRECTED MUTAGENESIS AND X-RAY CRYSTALLOGRAPHY | ||||||
![]() | FERREDOXIN-NADP+ REDUCTASE | ||||||
![]() | OXIDOREDUCTASE (NADP+(A) / FERREDOXIN(A)) | ||||||
Function / homology | ![]() chloroplast thylakoid membrane protein complex / ferredoxin-NADP+ reductase / ferredoxin-NADP+ reductase activity / NADPH dehydrogenase activity / chloroplast stroma / photosynthesis / electron transport chain Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Bruns, C.M. / Karplus, P.A. | ||||||
![]() | ![]() Title: Involvement of serine 96 in the catalytic mechanism of ferredoxin-NADP+ reductase: structure--function relationship as studied by site-directed mutagenesis and X-ray crystallography. Authors: Aliverti, A. / Bruns, C.M. / Pandini, V.E. / Karplus, P.A. / Vanoni, M.A. / Curti, B. / Zanetti, G. #1: ![]() Title: Refined Crystal Structure of Spinach Ferredoxin Reductase at 1.7 Angstroms Resolution: Oxidized, Reduced, and 2'-Phospho-5'-AMP Bound States Authors: Bruns, C.M. / Karplus, P.A. #2: ![]() Title: Atomic Structure of Ferredoxin-Nadp+ Reductase: Prototype for a Structurally Novel Flavoenzyme Family Authors: Karplus, P.A. / Daniels, M.J. / Herriott, J.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 79.4 KB | Display | ![]() |
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PDB format | ![]() | 58.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 787.7 KB | Display | ![]() |
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Full document | ![]() | 800.3 KB | Display | |
Data in XML | ![]() | 17.5 KB | Display | |
Data in CIF | ![]() | 25 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 150 | ||||||||
Components on special symmetry positions |
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Components
#1: Protein | Mass: 35441.797 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-PO4 / |
#3: Chemical | ChemComp-SO4 / |
#4: Chemical | ChemComp-FAD / |
#5: Water | ChemComp-HOH / |
Compound details | COMPND PH 4.6, RESIDUE 96 MUTATED FROM SERINE TO VALINE, RECOMBINANT VARIANT WITH PHENYLALANINE AT ...COMPND PH 4.6, RESIDUE 96 MUTATED FROM SERINE TO VALINE, RECOMBINAN |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.29 % | ||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 4.5 / Method: unknown | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5 |
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Detector | Type: SDMS / Date: Jun 15, 1994 |
Radiation | Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5 Å / Relative weight: 1 |
Reflection | Redundancy: 4.8 % / Rmerge(I) obs: 0.088 |
Reflection | *PLUS Num. obs: 22757 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.088 |
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Processing
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Refinement | Highest resolution: 2 Å / σ(F): 0 /
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Refinement step | Cycle: LAST / Highest resolution: 2 Å
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Refine LS restraints |
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