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基本情報
登録情報 | データベース: PDB / ID: 1fpg | ||||||
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タイトル | STRUCTURAL ASPECTS OF THE ALLOSTERIC INHIBITION OF FRUCTOSE-1,6-BISPHOSPHATASE BY AMP: THE BINDING OF BOTH THE SUBSTRATE ANALOGUE 2,5-ANHYDRO-D-GLUCITOL-1,6-BISPHOSPHATE AND CATALYTIC METAL IONS MONITORED BY X-RAY CRYSTALLOGRAPHY | ||||||
![]() | FRUCTOSE 1,6-BISPHOSPHATASE | ||||||
![]() | HYDROLASE (PHOSPHORIC MONOESTER) | ||||||
機能・相同性 | ![]() Gluconeogenesis / sucrose biosynthetic process / fructose-bisphosphatase / fructose 1,6-bisphosphate 1-phosphatase activity / cellular response to magnesium ion / negative regulation of Ras protein signal transduction / fructose 6-phosphate metabolic process / fructose metabolic process / monosaccharide binding / fructose 1,6-bisphosphate metabolic process ...Gluconeogenesis / sucrose biosynthetic process / fructose-bisphosphatase / fructose 1,6-bisphosphate 1-phosphatase activity / cellular response to magnesium ion / negative regulation of Ras protein signal transduction / fructose 6-phosphate metabolic process / fructose metabolic process / monosaccharide binding / fructose 1,6-bisphosphate metabolic process / negative regulation of glycolytic process / regulation of gluconeogenesis / AMP binding / dephosphorylation / gluconeogenesis / negative regulation of cell growth / cellular response to xenobiotic stimulus / identical protein binding / metal ion binding / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() | ||||||
![]() | Villeret, V. / Huang, S. / Zhang, Y. / Lipscomb, W.N. | ||||||
![]() | ![]() タイトル: Structural aspects of the allosteric inhibition of fructose-1,6-bisphosphatase by AMP: the binding of both the substrate analogue 2,5-anhydro-D-glucitol 1,6-bisphosphate and catalytic ...タイトル: Structural aspects of the allosteric inhibition of fructose-1,6-bisphosphatase by AMP: the binding of both the substrate analogue 2,5-anhydro-D-glucitol 1,6-bisphosphate and catalytic metal ions monitored by X-ray crystallography. 著者: Villeret, V. / Huang, S. / Zhang, Y. / Lipscomb, W.N. #1: ![]() タイトル: Toward a Mechanism for the Allosteric Transition of Pig Kidney Fructose-1,6-Bisphosphatase 著者: Zhang, Y. / Liang, J.-Y. / Huang, S. / Lipscomb, W.N. #2: ![]() タイトル: Crystallographic Studies of the Catalytic Mechanism of the Neutral Form of Fructose-1,6-Bisphosphatase 著者: Zhang, Y. / Liang, J.-Y. / Huang, S. / Ke, H. / Lipscomb, W.N. #3: ![]() タイトル: Conformational Transition of Fructose-1,6-Bisphosphatase: Structure Comparison between the AMP Complex (T Form) and the Fructose 6-Phosphate Complex (R Form) 著者: Ke, H. / Liang, J.-Y. / Zhang, Y. / Lipscomb, W.N. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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PDBx/mmCIF形式 | ![]() | 162.1 KB | 表示 | ![]() |
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PDB形式 | ![]() | 132.8 KB | 表示 | ![]() |
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その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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詳細 | THERE ARE TWO MONOMERS IN THE ASYMMETRIC UNIT, WHICH IS HALF THE MOLECULE. RESIDUES 1 - 8 AND 62 - 71 WERE OMITTED DUE TO A LACK OF ELECTRON DENSITY. |
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要素
#1: タンパク質 | 分子量: 36503.004 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() #2: 化合物 | ChemComp-MN / #3: 糖 | #4: 化合物 | #5: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.79 Å3/Da / 溶媒含有率: 55.85 % | ||||||||||||||||||||||||||||||||||||||||||
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結晶化 | pH: 7.4 / 詳細: pH 7.4, ROOM TEMPERATURE, COCRYSTALLIZATION. | ||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 手法: microdialysis / 詳細: Ke, H., (1989) J. Mol. Biol., 212, 513. | ||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射 | 散乱光タイプ: x-ray |
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放射波長 | 相対比: 1 |
反射 | Num. obs: 36031 / % possible obs: 92.7 % |
反射 | *PLUS 最高解像度: 2.3 Å / Num. measured all: 79208 / Rmerge(I) obs: 0.074 |
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解析
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精密化 | 解像度: 2.3→8 Å / σ(F): 2 /
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原子変位パラメータ | Biso mean: 28 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2.3→8 Å
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拘束条件 |
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拘束条件 | *PLUS タイプ: x_angle_d / Dev ideal: 1.86 |