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Open data
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Basic information
Entry | Database: PDB / ID: 1fm0 | ||||||
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Title | MOLYBDOPTERIN SYNTHASE (MOAD/MOAE) | ||||||
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![]() | TRANSFERASE / MOLYBDENUM COFACTOR BIOSYNTHESIS | ||||||
Function / homology | ![]() molybdopterin adenylyltransferase complex / molybdopterin synthase complex / molybdopterin synthase / molybdopterin synthase activity / Mo-molybdopterin cofactor biosynthetic process / nucleotide binding / protein homodimerization activity / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Rudolph, M.J. / Wuebbens, M.M. / Rajagolpalan, K.V. / Schindelin, H. | ||||||
![]() | ![]() Title: Crystal structure of molybdopterin synthase and its evolutionary relationship to ubiquitin activation. Authors: Rudolph, M.J. / Wuebbens, M.M. / Rajagopalan, K.V. / Schindelin, H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 61.1 KB | Display | ![]() |
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PDB format | ![]() | 44.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Details | The biological assembly is a heteroteteramer consisting of two alpha subunits and two beta subunits where the alpha-beta heterodimer falls on a crystallographic twofold axis of symmetry to generate the heterotetramer |
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Components
#1: Protein | Mass: 8764.880 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Protein | Mass: 17003.020 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 46.12 % | ||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion / pH: 7.5 Details: Sodium chloride, Hepes , pH 7.5, VAPOR DIFFUSION, temperature 295K | ||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jan 1, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 1.45→50 Å / Num. all: 426992 / Num. obs: 43689 / % possible obs: 95.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.5 % / Biso Wilson estimate: 19.3 Å2 / Rmerge(I) obs: 0.067 / Net I/σ(I): 27.9 |
Reflection shell | Resolution: 1.45→1.51 Å / Redundancy: 4 % / Rmerge(I) obs: 0.454 / Num. unique all: 45862 / % possible all: 89.5 |
Reflection | *PLUS % possible obs: 95.6 % / Num. measured all: 426992 / Rmerge(I) obs: 0.054 |
Reflection shell | *PLUS % possible obs: 89.5 % / Mean I/σ(I) obs: 1.9 |
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Processing
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Refinement | Resolution: 1.45→10 Å / SU B: 2.77415 / SU ML: 0.05579 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.12845 / ESU R Free: 0.09003 / Stereochemistry target values: Murshudov (REFMAC5) Details: Individual anisotropic temperature factors were refined.
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Displacement parameters | Biso mean: 19.41 Å2
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Refinement step | Cycle: LAST / Resolution: 1.45→10 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 0 / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |