+Open data
-Basic information
Entry | Database: PDB / ID: 1fhh | ||||||
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Title | X-RAY CRYSTAL STRUCTURE OF OXIDIZED RUBREDOXIN | ||||||
Components | RUBREDOXIN | ||||||
Keywords | ELECTRON TRANSPORT / oxidized / Cp Rd / rubredoxin | ||||||
Function / homology | Function and homology information alkane catabolic process / electron transfer activity / iron ion binding Similarity search - Function | ||||||
Biological species | Clostridium pasteurianum (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.5 Å | ||||||
Authors | Min, T. / Ergenekan, C.E. / Eidsness, M.K. / Ichiye, T. / Kang, C. | ||||||
Citation | Journal: Protein Sci. / Year: 2001 Title: Leucine 41 is a gate for water entry in the reduction of Clostridium pasteurianum rubredoxin. Authors: Min, T. / Ergenekan, C.E. / Eidsness, M.K. / Ichiye, T. / Kang, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fhh.cif.gz | 22.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fhh.ent.gz | 13.9 KB | Display | PDB format |
PDBx/mmJSON format | 1fhh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fhh_validation.pdf.gz | 366.8 KB | Display | wwPDB validaton report |
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Full document | 1fhh_full_validation.pdf.gz | 369.2 KB | Display | |
Data in XML | 1fhh_validation.xml.gz | 3 KB | Display | |
Data in CIF | 1fhh_validation.cif.gz | 4.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fh/1fhh ftp://data.pdbj.org/pub/pdb/validation_reports/fh/1fhh | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 6051.611 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: OXIDIZED / Source: (gene. exp.) Clostridium pasteurianum (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P00268 |
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#2: Chemical | ChemComp-FE / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 7 |
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-Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41.48 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 2M ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 103 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL1-5 / Wavelength: 1.072 |
Detector | Type: FUJI / Detector: IMAGE PLATE / Date: Jun 27, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.072 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→10 Å / Num. all: 9328 / Num. obs: 8577 / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.059 |
Reflection shell | Resolution: 1.5→10 Å / Rmerge(I) obs: 0.059 / Num. unique all: 9328 / % possible all: 96 |
-Processing
Software |
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Refinement | Resolution: 1.5→10 Å / σ(I): 2
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Refinement step | Cycle: LAST / Resolution: 1.5→10 Å
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.5 Å / Lowest resolution: 10 Å | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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