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- PDB-1f7g: SOLUTION STRUCTURE OF THE RNASE P RNA (M1 RNA) P4 STEM C70U MUTAN... -
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Basic information
Entry | Database: PDB / ID: 1f7g | ||||||
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Title | SOLUTION STRUCTURE OF THE RNASE P RNA (M1 RNA) P4 STEM C70U MUTANT OLIGORIBONUCLEOTIDE, ENSEMBLE OF 17 STRUCTURES | ||||||
![]() | RNASE P RNA RIBOZYME, P4 DOMAIN | ||||||
![]() | RNA / RIBONUCLEASE P / RIBOZYME / TRANSFER RNA PROCESSING / P4 STEM / C70U MUTANT / METAL BINDING SITE | ||||||
Function / homology | RNA / RNA (> 10)![]() | ||||||
Method | SOLUTION NMR / restrained molecular dynamics; simulated annealing | ||||||
![]() | Schmitz, M. / Tinoco Jr., I. | ||||||
![]() | ![]() Title: Solution structure and metal-ion binding of the P4 element from bacterial RNase P RNA. Authors: Schmitz, M. / Tinoco Jr., I. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 278.7 KB | Display | ![]() |
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PDB format | ![]() | 235.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 311.5 KB | Display | ![]() |
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Full document | ![]() | 413.6 KB | Display | |
Data in XML | ![]() | 10.9 KB | Display | |
Data in CIF | ![]() | 18.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1f6xC ![]() 1f6zC ![]() 1f78C ![]() 1f79C ![]() 1f7fC ![]() 1f7hC ![]() 1f7iC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 8634.127 Da / Num. of mol.: 1 / Fragment: P4 STEM / Mutation: C70U / Source method: obtained synthetically Details: synthesized from DNA oligonucleotide template by T7 RNA polymerase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques and 13C and 31P heteronuclear techniques at natural abundance |
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Sample preparation
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: restrained molecular dynamics; simulated annealing / Software ordinal: 1 Details: The structures are based on a total of 268 NOE derived distance constraints, 171 dihedral restraints and 49 distance restraints from hydrogen bonds. The 17 structures with lowest NOE and ...Details: The structures are based on a total of 268 NOE derived distance constraints, 171 dihedral restraints and 49 distance restraints from hydrogen bonds. The 17 structures with lowest NOE and dihedral angle violation energies are presented | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 50 / Conformers submitted total number: 17 |