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Yorodumi- PDB-1f2r: NMR STRUCTURE OF THE HETERODIMERIC COMPLEX BETWEEN CAD DOMAINS OF... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1f2r | ||||||
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Title | NMR STRUCTURE OF THE HETERODIMERIC COMPLEX BETWEEN CAD DOMAINS OF CAD AND ICAD | ||||||
Components |
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Keywords | DNA BINDING PROTEIN / ALPHA-BETA ROLL / PROTEIN-PROTEIN COMPLEX | ||||||
Function / homology | Function and homology information negative regulation of deoxyribonuclease activity / deoxyribonuclease inhibitor activity / Apoptosis induced DNA fragmentation / negative regulation of apoptotic DNA fragmentation / DNA nuclease activity / Hydrolases / apoptotic chromosome condensation / apoptotic DNA fragmentation / nuclease activity / negative regulation of execution phase of apoptosis ...negative regulation of deoxyribonuclease activity / deoxyribonuclease inhibitor activity / Apoptosis induced DNA fragmentation / negative regulation of apoptotic DNA fragmentation / DNA nuclease activity / Hydrolases / apoptotic chromosome condensation / apoptotic DNA fragmentation / nuclease activity / negative regulation of execution phase of apoptosis / DNA catabolic process / thymocyte apoptotic process / chaperone-mediated protein folding / protein folding chaperone / DNA endonuclease activity / disordered domain specific binding / regulation of apoptotic process / positive regulation of apoptotic process / protein domain specific binding / chromatin / nucleolus / enzyme binding / protein-containing complex / DNA binding / nucleoplasm / identical protein binding / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Otomo, T. / Sakahira, H. / Uegaki, K. / Nagata, S. / Yamazaki, T. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2000 Title: Structure of the heterodimeric complex between CAD domains of CAD and ICAD. Authors: Otomo, T. / Sakahira, H. / Uegaki, K. / Nagata, S. / Yamazaki, T. #1: Journal: J.Mol.Biol. / Year: 2000 Title: Structure of the CAD Domain of Caspase-Activated DNase and Interaction with the CAD Domain of its Inhibitor. Authors: Uegaki, K. / Otomo, T. / Sakahira, H. / Shimizu, M. / Yumoto, N. / Kyogoku, Y. / Nagata, S. / Yamazaki, T. #2: Journal: Nature / Year: 1998 Title: A Caspase-Activated DNase that Degrades DNA During Apoptosis, and its Inhibitor ICAD. Authors: Enari, M. / Sakahira, H. / Yokoyama, H. / Okawa, K. / Iwamatsu, A. / Nagata, S. #3: Journal: Nature / Year: 1998 Title: Cleavage of CAD Inhibitor in CAD Activation and DNA Degradation During Apoptosis. Authors: Sakahira, H. / Enari, M. / Nagata, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f2r.cif.gz | 74.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f2r.ent.gz | 57.4 KB | Display | PDB format |
PDBx/mmJSON format | 1f2r.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f2/1f2r ftp://data.pdbj.org/pub/pdb/validation_reports/f2/1f2r | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9613.200 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DOMAIN (CAD DOMAIN), RESIDUES 1-87 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Plasmid: PET32 / Production host: Escherichia coli (E. coli) / References: UniProt: O54788 |
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#2: Protein | Mass: 11076.416 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DOMAIN (CAD DOMAIN), RESIDUES 1-100 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Plasmid: PET32 / Production host: Escherichia coli (E. coli) / References: UniProt: O54786 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 0 / pH: 6.4 / Pressure: ambient / Temperature: 308 K | |||||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: the structures are based on a total of 2646 restraints, 2503 are NOE-derived distance constraints, 93 dihedral angle restraints,50 distance restraints from hydrogen bonds. | ||||||||||||
NMR ensemble | Conformers submitted total number: 1 |