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- PDB-1f2r: NMR STRUCTURE OF THE HETERODIMERIC COMPLEX BETWEEN CAD DOMAINS OF... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1f2r | ||||||
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Title | NMR STRUCTURE OF THE HETERODIMERIC COMPLEX BETWEEN CAD DOMAINS OF CAD AND ICAD | ||||||
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![]() | DNA BINDING PROTEIN / ALPHA-BETA ROLL / PROTEIN-PROTEIN COMPLEX | ||||||
Function / homology | ![]() negative regulation of deoxyribonuclease activity / deoxyribonuclease inhibitor activity / Apoptosis induced DNA fragmentation / negative regulation of apoptotic DNA fragmentation / DNA nuclease activity / Hydrolases / apoptotic chromosome condensation / apoptotic DNA fragmentation / nuclease activity / negative regulation of execution phase of apoptosis ...negative regulation of deoxyribonuclease activity / deoxyribonuclease inhibitor activity / Apoptosis induced DNA fragmentation / negative regulation of apoptotic DNA fragmentation / DNA nuclease activity / Hydrolases / apoptotic chromosome condensation / apoptotic DNA fragmentation / nuclease activity / negative regulation of execution phase of apoptosis / thymocyte apoptotic process / chaperone-mediated protein folding / protein folding chaperone / DNA endonuclease activity / disordered domain specific binding / regulation of apoptotic process / positive regulation of apoptotic process / protein domain specific binding / chromatin / nucleolus / enzyme binding / protein-containing complex / DNA binding / nucleoplasm / identical protein binding / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Otomo, T. / Sakahira, H. / Uegaki, K. / Nagata, S. / Yamazaki, T. | ||||||
![]() | ![]() Title: Structure of the heterodimeric complex between CAD domains of CAD and ICAD. Authors: Otomo, T. / Sakahira, H. / Uegaki, K. / Nagata, S. / Yamazaki, T. #1: ![]() Title: Structure of the CAD Domain of Caspase-Activated DNase and Interaction with the CAD Domain of its Inhibitor. Authors: Uegaki, K. / Otomo, T. / Sakahira, H. / Shimizu, M. / Yumoto, N. / Kyogoku, Y. / Nagata, S. / Yamazaki, T. #2: ![]() Title: A Caspase-Activated DNase that Degrades DNA During Apoptosis, and its Inhibitor ICAD. Authors: Enari, M. / Sakahira, H. / Yokoyama, H. / Okawa, K. / Iwamatsu, A. / Nagata, S. #3: ![]() Title: Cleavage of CAD Inhibitor in CAD Activation and DNA Degradation During Apoptosis. Authors: Sakahira, H. / Enari, M. / Nagata, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 74.7 KB | Display | ![]() |
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PDB format | ![]() | 57.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 245.5 KB | Display | ![]() |
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Full document | ![]() | 245.2 KB | Display | |
Data in XML | ![]() | 7.1 KB | Display | |
Data in CIF | ![]() | 9.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 9613.200 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DOMAIN (CAD DOMAIN), RESIDUES 1-87 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 11076.416 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DOMAIN (CAD DOMAIN), RESIDUES 1-100 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details |
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Sample conditions | Ionic strength: 0 / pH: 6.4 / Pressure: ambient / Temperature: 308 K | |||||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: the structures are based on a total of 2646 restraints, 2503 are NOE-derived distance constraints, 93 dihedral angle restraints,50 distance restraints from hydrogen bonds. | ||||||||||||
NMR ensemble | Conformers submitted total number: 1 |