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- PDB-1f0o: PVUII ENDONUCLEASE/COGNATE DNA COMPLEX (GLUTARALDEHYDE-CROSSLINKE... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1f0o | ||||||
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Title | PVUII ENDONUCLEASE/COGNATE DNA COMPLEX (GLUTARALDEHYDE-CROSSLINKED CRYSTAL) AT PH 7.5 WITH TWO CALCIUM IONS AT EACH ACTIVE SITE | ||||||
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![]() | hydrolase/DNA / PROTEIN-DNA COMPLEX / ENDONUCLEASE TYPE II / RESTRICTION ENZYME / Catalytic Metal visualization / hydrolase-DNA COMPLEX | ||||||
Function / homology | ![]() type II site-specific deoxyribonuclease / type II site-specific deoxyribonuclease activity / DNA restriction-modification system / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Horton, J.R. / Cheng, X. | ||||||
![]() | ![]() Title: PvuII endonuclease contains two calcium ions in active sites. Authors: Horton, J.R. / Cheng, X. #1: ![]() Title: Asp34 of PvuII Endonuclease is directly involved in DNA minor groove recognition and indirectly involved in catalysis Authors: Horton, J.R. / Nastri, H.G. / Riggs, P.D. / Cheng, X. #2: ![]() Title: How is modification of the DNA substrate recognized by the PvuII restriction endonuclease? Authors: Horton, J.R. / Bonventre, J. / Cheng, X. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 94.5 KB | Display | ![]() |
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PDB format | ![]() | 68.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | The biological assembly is a dimer consisting of protein chain A and chain B and double strained DNA consisting of chains C and D with two CA+2 at each monomeric active site. |
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Components
#1: DNA chain | Mass: 3967.585 Da / Num. of mol.: 2 / Source method: obtained synthetically Details: SELF-ANNEALING OLIGONUCLEOTIDE CONTAINING COGNATE SIX BASE PAIR SEQUENCE #2: Protein | Mass: 18370.992 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P23657, type II site-specific deoxyribonuclease #3: Chemical | ChemComp-CA / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.55 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: PEG 4000, SODIUM ACETATE, CaCL2, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 16 ℃Details: Balendiran, K., (1994) Proteins Struct.Funct.Genet., 19, 77. | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Feb 14, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→30 Å / Num. all: 13150 / Num. obs: 13150 / % possible obs: 98.9 % / Observed criterion σ(I): -3 / Redundancy: 4.7 % / Biso Wilson estimate: 23.7 Å2 / Rmerge(I) obs: 0.057 / Net I/σ(I): 20.1 |
Reflection shell | Resolution: 2.5→2.54 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.332 / % possible all: 96.5 |
Reflection | *PLUS Num. measured all: 61937 |
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Processing
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Refinement | Resolution: 2.5→30 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.5→30 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 30 Å / σ(F): 0 / % reflection Rfree: 10 % / Rfactor obs: 0.205 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_deg / Dev ideal: 1.3 |