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- PDB-1eww: SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEG... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1eww | ||||||
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Title | SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS | ||||||
![]() | ANTIFREEZE PROTEIN | ||||||
![]() | ANTIFREEZE PROTEIN / beta-helix / ice / insect | ||||||
Function / homology | Choristoneura fumiferana antifreeze / Insect antifreeze protein superfamily / Choristoneura fumiferana antifreeze protein (CfAFP) / Insect antifreeze protein / UDP N-Acetylglucosamine Acyltransferase; domain 1 / 3 Solenoid / Mainly Beta / Thermal hysteresis protein![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Graether, S.P. / Kuiper, M.J. / Gagne, S.M. / Walker, V.K. / Jia, Z. / Sykes, B.D. / Davies, P.L. | ||||||
![]() | ![]() Title: Beta-helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect. Authors: Graether, S.P. / Kuiper, M.J. / Gagne, S.M. / Walker, V.K. / Jia, Z. / Sykes, B.D. / Davies, P.L. #1: ![]() Title: The Antifreeze Potential of the Spruce Budworm Thermal Hysteresis Protein. Authors: Tyshenko, M.G. / Doucet, D. / Davies, P.L. / Walker, V.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 478 KB | Display | ![]() |
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PDB format | ![]() | 412.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 342.3 KB | Display | ![]() |
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Full document | ![]() | 538.4 KB | Display | |
Data in XML | ![]() | 49.8 KB | Display | |
Data in CIF | ![]() | 74.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 9068.034 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Tissue fraction: HEMOLYMPH / Plasmid: PET21 / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
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Sample preparation
Details | Contents: 1: U-15N/U-13C spruce budworm antifreeze protein, 18 mg/mL, 2: U-15N spruce budworm antifreeze protein, 18 mg/mL Solvent system: 90% H2O, 10% D20 |
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Sample conditions | Ionic strength: 0 / pH: 5.5 / Pressure: ambient / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: 1014 NOE-derived distance constraints, 88 dihedral angle restraints, 59 carbon chemical shift restraints | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 |