+Open data
-Basic information
Entry | Database: PDB / ID: 1eur | ||||||
---|---|---|---|---|---|---|---|
Title | SIALIDASE | ||||||
Components | SIALIDASE | ||||||
Keywords | HYDROLASE / NEURAMINIDASE / SIALIDASE | ||||||
Function / homology | Function and homology information ganglioside catabolic process / oligosaccharide catabolic process / exo-alpha-(2->3)-sialidase activity / exo-alpha-(2->6)-sialidase activity / exo-alpha-(2->8)-sialidase activity / exo-alpha-sialidase / intracellular membrane-bounded organelle / extracellular region / membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Micromonospora viridifaciens (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.82 Å | ||||||
Authors | Gaskell, A. / Crennell, S.J. / Taylor, G.L. | ||||||
Citation | Journal: Structure / Year: 1995 Title: The three domains of a bacterial sialidase: a beta-propeller, an immunoglobulin module and a galactose-binding jelly-roll. Authors: Gaskell, A. / Crennell, S. / Taylor, G. #1: Journal: J.Mol.Biol. / Year: 1992 Title: Crystallization and Preliminary Crystallographic Study of Neuraminidase from Micromonospora Viridifaciens Authors: Taylor, G. / Dineley, L. / Glowka, M. / Laver, G. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1eur.cif.gz | 85.3 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1eur.ent.gz | 63 KB | Display | PDB format |
PDBx/mmJSON format | 1eur.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1eur_validation.pdf.gz | 363.7 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1eur_full_validation.pdf.gz | 370.8 KB | Display | |
Data in XML | 1eur_validation.xml.gz | 8.9 KB | Display | |
Data in CIF | 1eur_validation.cif.gz | 14.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/eu/1eur ftp://data.pdbj.org/pub/pdb/validation_reports/eu/1eur | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 38892.703 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: 41KD FORM / Source: (natural) Micromonospora viridifaciens (bacteria) / References: UniProt: Q02834, exo-alpha-sialidase |
---|---|
#2: Water | ChemComp-HOH / |
Compound details | THE NANH_MICVI SEQUENCE REPRESENTS A 68KD PROTEIN. THE 42KD NEURAMINIDASE GIVEN HERE BELONGS TO THE ...THE NANH_MICVI SEQUENCE REPRESENTS |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 38.5 % |
---|---|
Crystal | *PLUS |
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / Details: Taylor, G., (1992) J.Mol.Biol., 225, 1135. / PH range low: 5.5 / PH range high: 5 |
Components of the solutions | *PLUS Conc.: 8 % / Common name: PEG3350 |
-Data collection
Diffraction source | Wavelength: 1.5418 |
---|---|
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 29469 / % possible obs: 93.4 % / Observed criterion σ(I): 1 / Redundancy: 5.6 % / Rmerge(I) obs: 0.055 |
Reflection | *PLUS Highest resolution: 1.82 Å / Num. measured all: 168296 |
Reflection shell | *PLUS Highest resolution: 1.82 Å / Lowest resolution: 2 Å / % possible obs: 71.7 % / Rmerge(I) obs: 0.094 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.82→6 Å / σ(F): 0 /
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 13.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.15 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.82→6 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
|