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Yorodumi- PDB-1eu8: STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1eu8 | |||||||||
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Title | STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS | |||||||||
Components | TREHALOSE/MALTOSE BINDING PROTEIN | |||||||||
Keywords | SUGAR BINDING PROTEIN / TREHALOSE-MALTOSE BINDING PROTEIN / PROTEIN-CARBOHYDRATE COMPLEX / MBP 2 FOLD / ABC TRANSPORTER FOLD / THERMOPHILIC PROTEIN | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Thermococcus litoralis (archaea) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.9 Å | |||||||||
Authors | Diez, J. / Diederichs, K. / Greller, G. / Horlacher, R. / Boos, W. / Welte, W. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 2001 Title: The crystal structure of a liganded trehalose/maltose-binding protein from the hyperthermophilic Archaeon Thermococcus litoralis at 1.85 A. Authors: Diez, J. / Diederichs, K. / Greller, G. / Horlacher, R. / Boos, W. / Welte, W. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1eu8.cif.gz | 102.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1eu8.ent.gz | 76.7 KB | Display | PDB format |
PDBx/mmJSON format | 1eu8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/eu/1eu8 ftp://data.pdbj.org/pub/pdb/validation_reports/eu/1eu8 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 46411.320 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermococcus litoralis (archaea) / Plasmid: PRHO1000 / Production host: Escherichia coli (E. coli) / References: UniProt: O51923, UniProt: Q7LYW7*PLUS | ||||
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#2: Polysaccharide | alpha-D-glucopyranose-(1-1)-alpha-D-glucopyranose / trehalose / | ||||
#3: Chemical | #4: Chemical | ChemComp-CL / #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45.28 % | |||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 25% POLYETHYLENE GLYCOL 4000, 0.2 M AMMONIUM SULFATE, 0.1 M NA-ACETATE, PROTEIN CONCENTRATION 6 mg/ml MIXED 1:1 WITH CRYSTALLIZATION SOLUTION, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: BW7B / Wavelength: 0.8439 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 12, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8439 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→20 Å / Num. all: 66464 / Num. obs: 66464 / % possible obs: 96.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.6 % / Biso Wilson estimate: 25.657 Å2 / Rmerge(I) obs: 0.079 / Net I/σ(I): 13.42 |
Reflection shell | Resolution: 1.85→1.9 Å / Redundancy: 2 % / Rmerge(I) obs: 0.337 / Num. unique all: 4665 / % possible all: 87.7 |
Reflection | *PLUS Num. obs: 67503 / % possible obs: 97.7 % / Rmerge(I) obs: 0.08 |
Reflection shell | *PLUS % possible obs: 87.4 % / Num. unique obs: 4665 |
-Processing
Software |
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Refinement | Resolution: 1.9→20 Å / Occupancy max: 1 / Occupancy min: 0.31 / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: ENGH & HUBER
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Solvent computation | Solvent model: CNS bulk solvent model used / Bsol: 50.6099 Å2 / ksol: 0.371351 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.53 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.9→20 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.85 Å | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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