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- PDB-1et0: CRYSTAL STRUCTURE OF AMINODEOXYCHORISMATE LYASE FROM ESCHERICHIA COLI -
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Open data
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Basic information
Entry | Database: PDB / ID: 1et0 | ||||||
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Title | CRYSTAL STRUCTURE OF AMINODEOXYCHORISMATE LYASE FROM ESCHERICHIA COLI | ||||||
![]() | 4-AMINO-4-DEOXYCHORISMATE LYASE | ||||||
![]() | LYASE / PSEUDO BETA BARREL | ||||||
Function / homology | ![]() aminodeoxychorismate lyase / 4-amino-4-deoxychorismate lyase activity / para-aminobenzoic acid biosynthetic process / folic acid biosynthetic process / tetrahydrofolate biosynthetic process / pyridoxal phosphate binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Nakai, T. / Mizutani, H. / Miyahara, I. / Hirotsu, K. / Takeda, S. / Jhee, K.H. / Yoshimura, T. / Esaki, N. | ||||||
![]() | ![]() Title: Three-dimensional structure of 4-amino-4-deoxychorismate lyase from Escherichia coli. Authors: Nakai, T. / Mizutani, H. / Miyahara, I. / Hirotsu, K. / Takeda, S. / Jhee, K.H. / Yoshimura, T. / Esaki, N. #1: ![]() Title: Characterization and Sequence of Escherichia coli pabC, the Gene Encoding Aminodeoxychorismate Lyase, a Pyridoxal Phosphate-Containing Enzyme Authors: Green, J.M. / Merkel, W.K. / Nichols, B.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 60.5 KB | Display | ![]() |
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PDB format | ![]() | 47.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 388.2 KB | Display | ![]() |
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Full document | ![]() | 391.7 KB | Display | |
Data in XML | ![]() | 7.1 KB | Display | |
Data in CIF | ![]() | 10.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Details | The biological assembly is a dimer constructed from chain A a symmetry partner generated by the two-fold. |
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Components
#1: Protein | Mass: 29745.031 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-PLP / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.61 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PEG 400, 2-propanol, sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Details: drop consists of equal volume of protein and reservoir solutions | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: FUJI / Detector: IMAGE PLATE / Date: Jan 20, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. all: 13365 / Num. obs: 12330 / % possible obs: 92.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7.14 % / Biso Wilson estimate: 21.4 Å2 / Rmerge(I) obs: 0.072 / Net I/σ(I): 30.9 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.135 / Num. unique all: 1110 / % possible all: 85 |
Reflection | *PLUS Num. measured all: 88073 |
Reflection shell | *PLUS % possible obs: 85 % |
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Processing
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Refinement | Resolution: 2.2→8 Å / σ(F): 2 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.2→8 Å
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Refine LS restraints |
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