- PDB-1esx: 1H, 15N AND 13C STRUCTURE OF THE HIV-1 REGULATORY PROTEIN VPR : C... -
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IDまたはキーワード:
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基本情報
登録情報
データベース: PDB / ID: 1esx
タイトル
1H, 15N AND 13C STRUCTURE OF THE HIV-1 REGULATORY PROTEIN VPR : COMPARISON WITH THE N-AND C-TERMINAL DOMAIN STRUCTURE, (1-51)VPR AND (52-96)VPR
要素
VPR PROTEIN
キーワード
VIRAL PROTEIN / helix / amphipatic / turn
機能・相同性
機能・相同性情報
symbiont-mediated arrest of host cell cycle during G2/M transition / symbiont-mediated activation of host apoptosis / protein homooligomerization / virion component / viral penetration into host nucleus / host cell / monoatomic ion transmembrane transport / host extracellular space / symbiont entry into host cell / DNA-templated transcription ...symbiont-mediated arrest of host cell cycle during G2/M transition / symbiont-mediated activation of host apoptosis / protein homooligomerization / virion component / viral penetration into host nucleus / host cell / monoatomic ion transmembrane transport / host extracellular space / symbiont entry into host cell / DNA-templated transcription / regulation of DNA-templated transcription / host cell nucleus 類似検索 - 分子機能
Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #4730 / Retroviral VpR/VpX protein / VPR/VPX protein / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Up-down Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest energy
代表モデル
モデル #20
fewest violations,lowest energy
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要素
#1: タンパク質
VPRPROTEIN / VIRAL PROTEIN OF REGULATION
分子量: 11411.905 Da / 分子数: 1 / 由来タイプ: 合成 詳細: This sequence has been obtained by solid phase synthesis. The sequence of this protein is naturally found in Human immunodeficiency virus type 1 (HIV-1). 参照: UniProt: Q73369
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D NOESY
1
2
1
DQF-COSY
1
3
1
HSQC (15N, 13C)
1
4
1
HSQC-NOESY (15N, 13C)
1
5
1
HSQC-TOCSY(15N, 13C)
NMR実験の詳細
Text: This structure was determined using standard 2D homonuclear techniques, 2D heteronuclear techniques and relaxation experiments
タイプ: Bruker DRX / 製造業者: Bruker / モデル: DRX / 磁場強度: 600 MHz
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解析
NMR software
名称
バージョン
開発者
分類
Discover
98
msi
構造決定
XwinNMR
2.1
bruker
collection
Discover
98
msi
精密化
XwinNMR
2.1
bruker
解析
精密化
手法: simulated annealing, molecular dynamics, minimisations ソフトェア番号: 1 詳細: The structure is based on a total of 1420 distance constraints
代表構造
選択基準: fewest violations,lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest energy 計算したコンフォーマーの数: 20 / 登録したコンフォーマーの数: 1