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Yorodumi- PDB-1esx: 1H, 15N AND 13C STRUCTURE OF THE HIV-1 REGULATORY PROTEIN VPR : C... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1esx | ||||||
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Title | 1H, 15N AND 13C STRUCTURE OF THE HIV-1 REGULATORY PROTEIN VPR : COMPARISON WITH THE N-AND C-TERMINAL DOMAIN STRUCTURE, (1-51)VPR AND (52-96)VPR | ||||||
Components | VPR PROTEIN | ||||||
Keywords | VIRAL PROTEIN / helix / amphipatic / turn | ||||||
Function / homology | Function and homology information symbiont-mediated arrest of host cell cycle during G2/M transition / monoatomic ion transmembrane transport / virion component / protein homooligomerization / viral penetration into host nucleus / host cell / host extracellular space / symbiont entry into host cell / cell cycle / DNA-templated transcription ...symbiont-mediated arrest of host cell cycle during G2/M transition / monoatomic ion transmembrane transport / virion component / protein homooligomerization / viral penetration into host nucleus / host cell / host extracellular space / symbiont entry into host cell / cell cycle / DNA-templated transcription / host cell nucleus / regulation of DNA-templated transcription Similarity search - Function | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics, minimisations | ||||||
Authors | Wecker, K. / Morellet, N. / Bouaziz, S. / Roques, B. | ||||||
Citation | Journal: Eur.J.Biochem. / Year: 2002 Title: NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol. Comparison with the Vpr N-terminal (1-51) and C-terminal (52-96) domains. Authors: Wecker, K. / Morellet, N. / Bouaziz, S. / Roques, B.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1esx.cif.gz | 41.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1esx.ent.gz | 30 KB | Display | PDB format |
PDBx/mmJSON format | 1esx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1esx_validation.pdf.gz | 347.4 KB | Display | wwPDB validaton report |
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Full document | 1esx_full_validation.pdf.gz | 348.5 KB | Display | |
Data in XML | 1esx_validation.xml.gz | 3.4 KB | Display | |
Data in CIF | 1esx_validation.cif.gz | 4.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/es/1esx ftp://data.pdbj.org/pub/pdb/validation_reports/es/1esx | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11411.905 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This sequence has been obtained by solid phase synthesis. The sequence of this protein is naturally found in Human immunodeficiency virus type 1 (HIV-1). References: UniProt: Q73369 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques, 2D heteronuclear techniques and relaxation experiments |
-Sample preparation
Details | Contents: 1 mM Vpr 15N, 13C ; 10 mM DTT ; HMDS ; 70 % H2O ; 30 % TFE Solvent system: 70 % H20, 30 % TFE | |||||||||||||||
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics, minimisations Software ordinal: 1 Details: The structure is based on a total of 1420 distance constraints | ||||||||||||||||||||
NMR representative | Selection criteria: fewest violations,lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 20 / Conformers submitted total number: 1 |