+Open data
-Basic information
Entry | Database: PDB / ID: 1eax | ||||||
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Title | Crystal structure of MTSP1 (matriptase) | ||||||
Components | SUPPRESSOR OF TUMORIGENICITY 14 | ||||||
Keywords | HYDROLASE / SERINE PROTEINASE / MATRIX DEGRADATION | ||||||
Function / homology | Function and homology information matriptase / epithelial cell morphogenesis involved in placental branching / Formation of the cornified envelope / keratinocyte differentiation / serine-type peptidase activity / neural tube closure / protein catabolic process / basolateral plasma membrane / external side of plasma membrane / serine-type endopeptidase activity ...matriptase / epithelial cell morphogenesis involved in placental branching / Formation of the cornified envelope / keratinocyte differentiation / serine-type peptidase activity / neural tube closure / protein catabolic process / basolateral plasma membrane / external side of plasma membrane / serine-type endopeptidase activity / proteolysis / extracellular space / plasma membrane Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.3 Å | ||||||
Authors | Friedrich, R. / Bode, W. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2002 Title: Catalytic Domain Structures of Mt-Sp1/Matriptase, a Matrix-Degrading Transmembrane Serine Proteinase. Authors: Friedrich, R. / Fuentes-Prior, P. / Ong, E. / Coombs, G. / Hunter, M. / Oehler, R. / Pierson, D. / Gonzalez, R. / Huber, R. / Bode, W. / Madison, E.L. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "AB" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 6-STRANDED BARREL THIS IS REPRESENTED BY A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1eax.cif.gz | 70.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1eax.ent.gz | 51.1 KB | Display | PDB format |
PDBx/mmJSON format | 1eax.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ea/1eax ftp://data.pdbj.org/pub/pdb/validation_reports/ea/1eax | HTTPS FTP |
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-Related structure data
Related structure data | 1eawC 1ekbS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 26463.756 Da / Num. of mol.: 1 / Fragment: CATALYTIC RESIDUES 615-855 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Production host: SACCHAROMYCES CEREVISIAE (brewer's yeast) References: UniProt: Q9Y5Y6, Hydrolases; Acting on peptide bonds (peptidases); Serine endopeptidases |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-BEN / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48 % | ||||||||||||||||||||||||
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Crystal grow | pH: 8 / Details: pH 8.00 | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 15, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.3→18 Å / Num. obs: 58805 / % possible obs: 96.2 % / Observed criterion σ(I): 2 / Redundancy: 3.3 % / Biso Wilson estimate: 12.1 Å2 / Rmerge(I) obs: 0.086 / Net I/σ(I): 4 |
Reflection shell | Resolution: 1.3→1.36 Å / Redundancy: 2.4 % / Rmerge(I) obs: 0.294 / Mean I/σ(I) obs: 1.3 / % possible all: 93.5 |
Reflection | *PLUS Highest resolution: 1.3 Å / Lowest resolution: 18 Å |
Reflection shell | *PLUS % possible obs: 93.5 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1EKB Resolution: 1.3→17.75 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 1648979.68 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Details: BULK SOLVENT MODEL USED
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 65 Å2 / ksol: 0.388634 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 13.7 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.3→17.75 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.3→1.38 Å / Rfactor Rfree error: 0.012 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 18 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.25 |