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- PDB-1e40: Tris/maltotriose complex of chimaeric amylase from B. amyloliquef... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1.0E+40 | |||||||||
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Title | Tris/maltotriose complex of chimaeric amylase from B. amyloliquefaciens and B. licheniformis at 2.2A | |||||||||
![]() | ALPHA-AMYLASE | |||||||||
![]() | HYDROLASE / AMYLASE / FAMILY 13 / MALTOTRIOSE / TRIS / COMPLEX | |||||||||
Function / homology | ![]() alpha-amylase / alpha-amylase activity / carbohydrate metabolic process / calcium ion binding / extracellular space Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Brzozowski, A.M. / Lawson, D.M. / Turkenburg, J.P. / Bisgaard-Frantzen, H. / Svendsen, A. / Borchert, T.V. / Dauter, Z. / Wilson, K.S. / Davies, G.J. | |||||||||
![]() | ![]() Title: Structural Analysis of a Chimeric Bacterial Alpha-Amylase. High Resolution Analysis of Native and Ligand Complexes Authors: Brzozowski, A.M. / Lawson, D.M. / Turkenburg, J.P. / Bisgaard-Frantzen, H. / Svendsen, A. / Borchert, T.V. / Dauter, Z. / Wilson, K.S. / Davies, G.J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 127.2 KB | Display | ![]() |
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PDB format | ![]() | 95.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 785 KB | Display | ![]() |
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Full document | ![]() | 792.1 KB | Display | |
Data in XML | ![]() | 26.3 KB | Display | |
Data in CIF | ![]() | 40 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1e3xSC ![]() 1e3zC ![]() 1e43C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein / Sugars , 2 types, 2 molecules A
#1: Protein | Mass: 55038.410 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: CHIMAERIC STRUCTURE CONSISTING OF RESIDUES 1 - 300 OF B. AMYLOLIQUEFACIENS AND RESIDUES 301 - 483 OF B. LICHENIFORMIS IN COMPLEX WITH TRIS AND MALTOTRIOSE Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Polysaccharide | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose / alpha-maltotriose |
-Non-polymers , 4 types, 545 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-CA / #4: Chemical | ChemComp-NA / | #5: Chemical | ChemComp-TRS / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: CRYSTALS WERE GROWN AT 18C USING THE HANGING DROP METHOD WITH 8-13% MONOMETHYL ETHER POLYETHYLENE GLYCOL 2000 OR 5000 AS PRECIPITANT. DROPS WERE BUFFERED WITH 0.1M TRIS/HCL PH 7.5 CONTAINING ...Details: CRYSTALS WERE GROWN AT 18C USING THE HANGING DROP METHOD WITH 8-13% MONOMETHYL ETHER POLYETHYLENE GLYCOL 2000 OR 5000 AS PRECIPITANT. DROPS WERE BUFFERED WITH 0.1M TRIS/HCL PH 7.5 CONTAINING 5MM CACL2 AND THE PROTEIN CONCENTRATION WAS 30-35MG/ML. CRYSTALS WERE THEN SOAKED IN 10MM MALTOTRIOSE SOLUTION TO OBTAIN THE COMPLEX. | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: RAXIS IIC / Detector: IMAGE PLATE / Date: Oct 15, 1994 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→30 Å / Num. obs: 23662 / % possible obs: 94 % / Redundancy: 5.2 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 33 |
Reflection shell | Resolution: 2.2→2.3 Å / Redundancy: 4.6 % / Rmerge(I) obs: 0.09 / Mean I/σ(I) obs: 13 / % possible all: 77 |
Reflection | *PLUS % possible obs: 94 % / Rmerge(I) obs: 0.05 |
Reflection shell | *PLUS % possible obs: 77 % / Rmerge(I) obs: 0.089 / Mean I/σ(I) obs: 13 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1E3X Resolution: 2.2→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.3 / ESU R Free: 0.22
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Displacement parameters | Biso mean: 12.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→20 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / Rfactor obs: 0.13 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |