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Open data
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Basic information
Entry | Database: PDB / ID: 1dlv | ||||||
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Title | BIOSYNTHETIC THIOLASE FROM ZOOGLOEA RAMIGERA IN COMPLEX WITH COA | ||||||
![]() | BIOSYNTHETIC THIOLASE | ||||||
![]() | TRANSFERASE / BIOSYNTHETIC THIOLASE / COA / TETRAMER | ||||||
Function / homology | ![]() poly-hydroxybutyrate biosynthetic process / acetyl-CoA C-acetyltransferase / acetyl-CoA C-acetyltransferase activity / fatty acid beta-oxidation / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Modis, Y. / Wierenga, R.K. | ||||||
![]() | ![]() Title: Crystallographic analysis of the reaction pathway of Zoogloea ramigera biosynthetic thiolase. Authors: Modis, Y. / Wierenga, R.K. #1: ![]() Title: A biosynthetic thiolase in complex with a reaction intermediate: the crystal structure provides new insights into the catalytic mechanism. Authors: Modis, Y. / Wierenga, R.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 311.4 KB | Display | ![]() |
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PDB format | ![]() | 249.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 75.4 KB | Display | |
Data in CIF | ![]() | 101.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 40255.914 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / #3: Chemical | ChemComp-COA / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.13 Å3/Da / Density % sol: 60.65 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 5 Details: AMMONIUM SULPHATE, LITHIUM SULPHATE, SODIUM ACETATE, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 294K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5 | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: AREA DETECTOR / Date: Feb 15, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.29→30 Å / Num. all: 93501 / Num. obs: 86956 / % possible obs: 93 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 2 / Redundancy: 4.3 % / Biso Wilson estimate: 14.6 Å2 / Rmerge(I) obs: 0.127 / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 2.29→2.45 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.274 / % possible all: 95.7 |
Reflection shell | *PLUS % possible obs: 95.7 % |
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Processing
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Refinement | Resolution: 2.29→30 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: ENGH & HUBER / Details: WEIGHTED SPARSE MATRIX LEAST SQUARES PROCEDURE
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Refinement step | Cycle: LAST / Resolution: 2.29→30 Å
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 30 Å / σ(F): 0 / Rfactor obs: 0.242 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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